Typing of mecD Islands in Genetically Diverse Methicillin-Resistant Macrococcus caseolyticus Strains from Cattle.

Schwendener, Sybille; Nigg, Aurélien; Collaud, Alexandra; Overesch, Gudrun; Kittl, Sonja; Phumthanakorn, Nathita; Perreten, Vincent (2019). Typing of mecD Islands in Genetically Diverse Methicillin-Resistant Macrococcus caseolyticus Strains from Cattle. Applied and environmental microbiology, 85(19) American Society for Microbiology 10.1128/AEM.01496-19

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Macrococcus caseolyticus belongs to the normal bacterial flora of dairy cows and does not usually cause disease. However, methicillin-resistant M. caseolyticus strains were isolated from bovine mastitis milk. These bacteria had acquired a chromosomal island (McRI
mecD
-1 or McRI
mecD
-2) carrying the methicillin resistance gene mecD To gain insight into the distribution of McRI
mecD
types in M. caseolyticus from cattle, 33 mecD-containing strains from Switzerland were characterized using molecular techniques, including multilocus sequence typing, antibiotic resistance gene identification, and PCR-based McRI
mecD
typing. In addition, the same genetic features were analyzed in 27 mecD-containing M. caseolyticus strains isolated from bovine bulk milk in England/Wales using publicly available whole-genome sequences. The 60 strains belonged to 24 different sequence types (STs), with strains belonging to ST5, ST6, ST21, and ST26 observed in both Switzerland and England/Wales. McRI
mecD
-1 was found in different STs from Switzerland (n = 19) and England/Wales (n = 4). McRI
mecD
-2 was only found in 7 strains from Switzerland, all of which belonged to ST6. A novel island, McRI
mecD
-3, which contains a complete mecD operon (mecD-mecR1m-mecIm [where the subscript m indicates Macrococcus]) combined with the left part of McRI
mecD
-2 and the right part of McRI
mecD
-1, was found in heterogeneous STs from both collections (Switzerland, n = 7; England/Wales, n = 21). Two strains from England/Wales carried a truncated McRI
mecD
-3. Phylogenetic analyses revealed no clustering of strains according to geographical origin or carriage of McRI
mecD
-1 and McRI
mecD
-3. Circular excisions were also detected for McRI
mecD
-1 and McRI
mecD
-3 by PCR. The analyses indicate that these islands are mobile and may spread by horizontal gene transfer between genetically diverse M. caseolyticus strains.IMPORTANCE Since its first description in 2017, the methicillin resistance gene mecD has been detected in M. caseolyticus strains from different cattle sources and countries. Our study provides new insights into the molecular diversity of mecD-carrying M. caseolyticus strains by using two approaches to characterize mecD elements: (i) multiplex PCR for molecular typing of McRI
mecD
and (ii) read mapping against reference sequences to identify McRI
mecD
types in silico In combination with multilocus sequence typing, this approach can be used for molecular characterization and surveillance of M. caseolyticus carrying mecD.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology > Molecular Bacterial Epidemiology and Infectiology
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Schwendener, Sybille; Nigg, Aurélien; Collaud, Alexandra; Overesch, Gudrun; Kittl, Sonja; Phumthanakorn, Nathita and Perreten, Vincent

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

1098-5336

Publisher:

American Society for Microbiology

Language:

English

Submitter:

Vincent Perreten

Date Deposited:

20 Sep 2019 11:00

Last Modified:

01 Apr 2020 02:30

Publisher DOI:

10.1128/AEM.01496-19

PubMed ID:

31375493

Uncontrolled Keywords:

McRImecD typing antibiotic resistance cattle chromosomal resistance island penicillin-binding protein 2a

BORIS DOI:

10.7892/boris.133373

URI:

https://boris.unibe.ch/id/eprint/133373

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