Multi-walled carbon nanotubes activate and shift polarization of pulmonary macrophages and dendritic cells in an in vivo model of chronic obstructive lung disease.

Beyeler, Seraina; Steiner, Selina; Wotzkow, Carlos; Tschanz, Stefan A.; Adhanom Sengal, Amanuel; Wick, Peter; Haenni, Beat; Alves, Marco P.; von Garnier, Christophe; Blank, Fabian (2019). Multi-walled carbon nanotubes activate and shift polarization of pulmonary macrophages and dendritic cells in an in vivo model of chronic obstructive lung disease. (In Press). Nanotoxicology, pp. 1-20. Taylor & Francis 10.1080/17435390.2019.1663954

Full text not available from this repository. (Request a copy)

With substantial progress of nanotechnology, there is rising concern about possible adverse health effects related to inhalation of nanomaterials, such as multi-walled carbon nanotubes (MWCNT). In particular, individuals with chronic respiratory disorders, such as chronic obstructive pulmonary disease (COPD), may potentially be more susceptible to adverse health effects related to inhaled MWCNT. Hazard assessment of such inhaled nanomaterials therefore requires timely clarification. This was assessed in this study using a mouse model of COPD by exposing animals to 0.08 µg/cm2 of MWCNT administered by intratracheal instillation. Treatment with MWCNT induced an accumulation of alveolar macrophages (AMφ) in bronchoalveolar lavage fluid (BALF) in COPD mice that increased from 24 h to 7 d. In COPD mice, MWCNT induced a dynamic shift in macrophage polarization as measured by expression of CD38 and CD206, and increased AMφ and lung parenchyma macrophage (LPMΦ) activation with upregulation of co-stimulatory markers CD40 and CD80. Moreover, MWCNT treatment increased the frequencies of pulmonary dendritic cells (DC), leading to an expansion of the CD11b+CD103- DC subset. Although MWCNT did not trigger lung functional or structural changes, they induced an increased expression of the muc5AC transcript in mice with COPD. Our data provide initial evidence that inhaled MWCNT affect the pulmonary mucosal immune system by altering the numbers, phenotype, and activation status of antigen-presenting cell populations. Extrapolating these in vivo mouse findings to human pulmonary MWCNT exposure, caution is warranted in limiting exposure when handling inhalable nanofibers.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)

UniBE Contributor:

Beyeler, Seraina Martina; Tschanz, Stefan A.; Haenni, Beat; Alves, Marco; von Garnier, Christophe and Blank, Fabian

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

1743-5404

Publisher:

Taylor & Francis

Language:

English

Submitter:

Heidi Lobsiger

Date Deposited:

15 Oct 2019 16:22

Last Modified:

15 Oct 2019 16:22

Publisher DOI:

10.1080/17435390.2019.1663954

PubMed ID:

31556347

Uncontrolled Keywords:

Multi-walled carbon nanotubes chronic obstructive pulmonary disease dendritic cells inflammation lung function macrophages morphometry neutrophils

URI:

https://boris.unibe.ch/id/eprint/133916

Actions (login required)

Edit item Edit item
Provide Feedback