Ventral Surgical Approach for an Intervertebral Disc Degeneration and Regeneration Model in Sheep Cervical Spine: Anatomic Technical Description, Strengths and Limitations.

Deml, Moritz C.; Benneker, Lorin M.; Schmid, Tanja; Sakai, Daisuke; Albers, Christoph E.; Hoppe, Sven; Zeiter, Stephan (2019). Ventral Surgical Approach for an Intervertebral Disc Degeneration and Regeneration Model in Sheep Cervical Spine: Anatomic Technical Description, Strengths and Limitations. Veterinary and comparative orthopaedics and traumatology : VCOT, 32(5), pp. 389-393. Thieme 10.1055/s-0039-1688988

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OBJECTIVE  Sheep are used as a large animal model for intervertebral disc research. However, for the ovine ventral surgical approach to the cervical disc, limited descriptions exist. We, therefore, give a detailed in vivo anatomical description of this approach in sheep for the use in intervertebral disc regeneration and degeneration models to increase the reproducibility of such interventions. MATERIALS AND METHODS  Eighteen female Swiss white alpine sheep, with an age range of 2 to 4 years, were used. A ventral surgical access was performed to establish defined intervertebral disc punch defect from cervical levels C3/4 to C5/6. Cervical levels C2/3 and C6/7 were used as negative controls. Intraoperative findings, radiographical controls and postoperative clinical follow-up were documented and collected for this clinical report. RESULTS  All sheep recovered rapidly from the surgical intervention. Two sheep developed wound seroma, which resorbed spontaneously. Two further sheep showed wound dehiscence within 3 days after surgery, which had to be revised. No clinical wound infections occurred and all sheep healed well and did not show any side effects related to the surgical procedure. CONCLUSION  The ventral surgical access to the ovine cervical spine is a safe and reliable procedure. The advantage of the cervical intervertebral disc is the easier surgical access and the increased disc height compared to the sheep lumbar spine. Since the ovine cervical intervertebral disc shows a high grade of similarities (e.g. molecular characteristics) compared to human discs, it is a promising degeneration and regeneration model for disc diseases.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery

UniBE Contributor:

Deml, Moritz Caspar; Benneker, Lorin Michael; Albers, Christoph and Hoppe, Sven

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0932-0814

Publisher:

Thieme

Language:

English

Submitter:

Kathrin Aeschlimann

Date Deposited:

30 Oct 2019 09:33

Last Modified:

04 Dec 2019 09:56

Publisher DOI:

10.1055/s-0039-1688988

PubMed ID:

31158895

BORIS DOI:

10.7892/boris.134154

URI:

https://boris.unibe.ch/id/eprint/134154

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