Häckel, Sonja; Zolfaghar, Mona; Du, Jie; Hoppe, Sven; Benneker, Lorin M.; Garstka, Nathalie; Peroglio, Marianna; Alini, Mauro; Grad, Sibylle; Yayon, Avner; Li, Zhen (2019). Fibrin-Hyaluronic Acid Hydrogel (RegenoGel) with Fibroblast Growth Factor-18 for In Vitro 3D Culture of Human and Bovine Nucleus Pulposus Cells. International journal of molecular sciences, 20(20) Molecular Diversity Preservation International MDPI 10.3390/ijms20205036
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We investigated the effects of a fibrin-hyaluronic acid hydrogel (FBG-HA) and fibroblast growth factor 18 (FGF-18) for nucleus pulposus (NP) regeneration. Healthy bovine (n = 4) and human degenerated NP cells (n = 4) were cultured for 14 days in FBG-HA hydrogel with FGF-18 (∆51-mutant or wild-type) in the culture medium. Gene expression, DNA content, and glycosaminoglycan (GAG) synthesis were evaluated on day 7 and 14. Additionally, histology was performed. Human NP cells cultured in FBG-HA hydrogel showed an increase in collagen type II (COL2) and carbonic anhydrase XII (CA12) gene expression after 14 or 7 days of culture, respectively. GAG release into the conditioned medium increased over 14 days. Healthy bovine NP cells showed increased gene expression of ACAN from day 7 to day 14. Wild type FGF-18 up-regulated CA12 gene expression of human NP cells. Histology revealed an increase of proteoglycan deposition upon FGF-18 stimulation in bovine but not in human NP cells. The FBG-HA hydrogel had a positive modulatory effect on human degenerated NP cells. Under the tested conditions, no significant effect of FGF-18 was observed on cell proliferation or GAG synthesis in human NP cells.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery |
UniBE Contributor: |
Häckel, Sonja, Hoppe, Sven, Benneker, Lorin Michael |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1661-6596 |
Publisher: |
Molecular Diversity Preservation International MDPI |
Language: |
English |
Submitter: |
Kathrin Aeschlimann |
Date Deposited: |
25 Oct 2019 14:32 |
Last Modified: |
05 Dec 2022 15:31 |
Publisher DOI: |
10.3390/ijms20205036 |
PubMed ID: |
31614494 |
Uncontrolled Keywords: |
fibrin-hyaluronic acid hydrogel fibroblast growth factor 18 nucleus pulposus cells |
BORIS DOI: |
10.7892/boris.134168 |
URI: |
https://boris.unibe.ch/id/eprint/134168 |
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