Membrane deformation and layer-by-layer peeling of giant vesicles induced by the pore-forming toxin pneumolysin

Drücker, Patrick; Iacovache, Ioan; Bachler, Simon; Zuber, Benoît; Babiychuk, Eduard B.; Dittrich, Petra S.; Draeger, Annette (2019). Membrane deformation and layer-by-layer peeling of giant vesicles induced by the pore-forming toxin pneumolysin. Biomaterials science, 7(9), pp. 3693-3705. Royal Society of Chemistry 10.1039/C9BM00134D

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Protein-membrane interactions that modify the shape of membranes are important for generating curvature, membrane deformation by protein-protein crowding or trafficking of vesicles. Giant vesicles represent a simplified but versatile model for biological membranes and are commonly employed for the study of lipid domains and permeation across compartments. In this study, we investigated the interaction of pneumolysin (PLY), a pore-forming toxin secreted by Streptococcus pneumoniae, with multilamellar and unilamellar membranes. It reveals an enlargement of membrane area due to the insertion of pores into the bilayer and protein-membrane aggregations that induce membrane deformation and wrinkling. Moreover, we demonstrate that PLY peel-off layers from multilamellar giant vesicles in a hitherto unknown layer-by-layer peeling mechanism, which reveals the structure and number of membrane lamellae. We employed microfluidic methods to capture giant vesicles and confocal laser scanning microscopy, transmission microscopy, dynamic light scattering and cryo-electron microscopy to disclose the structure of multilamellar vesicles. Based on our findings we suggest how back-to-back pore arrangements stabilize large PLY-membrane entities and that pore-displaced lipids possibly remain in the membrane.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Cell Biology
09 Interdisciplinary Units > Microscopy Imaging Center (MIC)

UniBE Contributor:

Drücker, Patrick; Iacovache, Mircea Ioan; Zuber, Benoît; Babiychuk, Eduard and Draeger, Annette

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2047-4830

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Benoît Zuber

Date Deposited:

11 Nov 2019 11:15

Last Modified:

11 Nov 2019 11:23

Publisher DOI:

10.1039/C9BM00134D

PubMed ID:

31187801

BORIS DOI:

10.7892/boris.134599

URI:

https://boris.unibe.ch/id/eprint/134599

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