Single-cell profiling identifies myeloid cell subsets with distinct fates during neuroinflammation.

Jordão, Marta Joana Costa; Sankowski, Roman; Brendecke, Stefanie M; Sagar, PhD; Locatelli, Giuseppe; Tai, Yi-Heng; Tay, Tuan Leng; Schramm, Eva; Armbruster, Stephan; Hagemeyer, Nora; Groß, Olaf; Mai, Dominic; Çiçek, Özgün; Falk, Thorsten; Kerschensteiner, Martin; Grün, Dominic; Prinz, Marco (2019). Single-cell profiling identifies myeloid cell subsets with distinct fates during neuroinflammation. Science, 363(6425) American Association for the Advancement of Science 10.1126/science.aat7554

Text eaat7554.full.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (4MB) | Request a copy

The innate immune cell compartment is highly diverse in the healthy central nervous system (CNS), including parenchymal and non-parenchymal macrophages. However, this complexity is increased in inflammatory settings by the recruitment of circulating myeloid cells. It is unclear which disease-specific myeloid subsets exist and what their transcriptional profiles and dynamics during CNS pathology are. Combining deep single-cell transcriptome analysis, fate mapping, in vivo imaging, clonal analysis, and transgenic mouse lines, we comprehensively characterized unappreciated myeloid subsets in several CNS compartments during neuroinflammation. During inflammation, CNS macrophage subsets undergo self-renewal, and random proliferation shifts toward clonal expansion. Last, functional studies demonstrated that endogenous CNS tissue macrophages are redundant for antigen presentation. Our results highlight myeloid cell diversity and provide insights into the brain's innate immune system.

Interest & Impact

Downloads

0 since deposited on 13 Nov 2019
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item