Tissue Response to a Porous Collagen Matrix Used for Soft Tissue Augmentation

Caballé-Serrano, Jordi; Zhang, Sophia; Ferrantino, Luca; Simion, Massimo; Chappuis, Vivianne; Bosshardt, Dieter (2019). Tissue Response to a Porous Collagen Matrix Used for Soft Tissue Augmentation. Materials, 12(22) Molecular Diversity Preservation International MDPI 10.3390/ma12223721

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Abstract: A short inflammatory phase and fast ingrowth of blood vessels and mesenchymal cells are essential for tissue integration of a biomaterial. Macrophages play a key role in this process. We investigated invasion of macrophages, blood vessels, and proliferating cells into a highly porous and volume-stable collagen matrix (VCMX) used for soft tissue augmentation around teeth and dental implants. The biomaterial was implanted in submucosal pouches in the canine maxilla, and the tissue response was analyzed at six different time points. Immunohistochemistry was done for proliferating cells (PCNA), macrophages (MAC387), multinucleated giant cells (CD86), and blood vessels (TGM2). Blood rapidly filled the VCMX pores. During the first week, MAC387+ cells populated the VCMX pores, blood vessels and PCNA+ cells invaded the VCMX, and CD86+ scattered cells were observed. At 15 days, MAC387+ cells were scanty, blood vessels had completely invaded the VCMX, the number of proliferating cells peaked, and fibroblasts appeared. At 30 days, MAC387+ were absent, the numbers of proliferating and CD86+ cells had declined, while blood vessel and fibroblast numbers were high. At 90 days, residual VCMX was well-integrated in soft connective tissue. In conclusion, the VCMX elicited a short inflammatory phase followed by rapid tissue integration.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research

UniBE Contributor:

Caballé Serrano, Jordi; Chappuis, Vivianne and Bosshardt, Dieter

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1996-1944

Publisher:

Molecular Diversity Preservation International MDPI

Language:

English

Submitter:

Caroline Balz

Date Deposited:

20 Nov 2019 15:49

Last Modified:

24 Nov 2019 02:45

Publisher DOI:

10.3390/ma12223721

PubMed ID:

31718004

Uncontrolled Keywords:

VCMX; Fibro-Gide; collagen matrix; immunohistochemistry; GTR; GBR; volume-stable collagen matrix

BORIS DOI:

10.7892/boris.134898

URI:

https://boris.unibe.ch/id/eprint/134898

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