Induction of (1 -> 3,1 -> 4)-beta-D-glucan hydrolases in leaves of dark-incubated barley seedlings

Roulin, Samuel; Buchala, Antony J.; Fincher, Geoffrey B. (2002). Induction of (1 -> 3,1 -> 4)-beta-D-glucan hydrolases in leaves of dark-incubated barley seedlings. Planta, 215(1), pp. 51-59. Springer-Verlag 10.1007/s00425-001-0721-1

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When seedlings of barley (Hordeum vulgare L.) were transferred from a natural light/dark cycle into darkness, (1-->3, 1-->4)-beta-D-glucan endohydrolase (EC 3.2.1.73) activity in leaf extracts increased 3- to 4-fold after 2 days. Activity decreased to normal levels within a day if the light/dark cycle was restored. Although there are two (1-->3,1-->4)-beta-D-glucan endohydrolase isoenzymes in barley, the increased enzyme activity in dark-grown seedlings was attributable entirely to increases in isoenzyme El. Northern hybridization analyses confirmed that mRNA transcripts encoding (1-->3,1-->4)-beta-D-glucan endohydrolase isoenzyme El accumulated in the leaves of dark-incubated seedlings;, no isoenzyme Ell mRNA was detected. Activity of beta-D-glucan glucohydrolases also increased 10-fold after 2 days of dark treatment. The latter, broad-specificity enzymes release glucose from (1-->3, 1-->4)-beta-D-glucans and from beta-D-Oligoglucosides released by D-glucan endohydrolases. Consistent with the activity patterns of these enzymes, the (1-->3,1-->4)-beta-D-glucan content of leaf cell walls decreased by about 30% when barley seedlings were transferred into darkness. Soluble sugars in the leaves decreased by about 60% during the same period. Because no measurable leaf elongation was detected during the various light/dark treatments, the enzymes were unlikely to be participating in wall loosening and cell elongation. Instead, the results suggest that cell wall (1-->3,1-->4)-beta-D-glucans can be re-mobilized in the non-elongating. dark-incubated leaves and the glucose so generated could serve as an energy source under conditions of sugar depletion.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Anoxia / Postanoxia [discontinued]
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0032-0935

Publisher:

Springer-Verlag

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

19 Nov 2019 14:21

Last Modified:

19 Nov 2019 14:21

Publisher DOI:

10.1007/s00425-001-0721-1

Uncontrolled Keywords:

cell wall; dark induction; (1 -> 3,1 -> 4)-beta-D-glucan; (1 -> 3, 1 -> 4)-beta-D-glucan endohydrolase; beta-D-glucan glucohydrolase; Hordeum (cell wall)

BORIS DOI:

10.7892/boris.134985

URI:

https://boris.unibe.ch/id/eprint/134985

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