Effect of the degree of conversion of resin-based composites on cytotoxicity, cell attachment, and gene expression.

Fujioka-Kobayashi, Masako; Miron, Richard J.; Lussi, Adrian; Gruber, Reinhard; Ilie, Nicoleta; Price, Richard Bengt; Schmalz, Gottfried (2019). Effect of the degree of conversion of resin-based composites on cytotoxicity, cell attachment, and gene expression. Dental materials, 35(8), pp. 1173-1193. Elsevier 10.1016/j.dental.2019.05.015

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OBJECTIVE This study investigated the influence of the degree of conversion (DC), resin-based composites (RBC) composition, and the effect of additional violet light from one light curing unit (LCU) on cell attachment/growth, eluate cytotoxicity, and gene expression. METHODS The effect of different DC of RBCs on human gingival fibroblasts (HGFs) when cultured directly onto cured RBCs, and when exposed afterwards to eluates in cell culture medium was examined. Venus® (RBC-V; Bis-GMA-based) and Venus Pearl® (RBC-P; TCD-DI-HEA and UDMA-based) were cured using a single emission peak (blue) light, Translux Wave®; TW and a dual emission peak (blue-violet) light, Translux 2 Wave®; T2W. To determine the value of the additional violet light from the T2W, exposure times and distances were adjusted to deliver similar radiant exposures (RE) from the blue region of both lights at five different RE levels from 1.5 J/cm² to 28.9 J/cm². RESULTS Both RBCs light-cured with the T2W at higher REs resulted in higher DC, increased cell adhesion and decreased eluate cytotoxicity. RBC-V induced greater cell adhesion, lower mRNA levels of pro-inflammatory markers, and higher mRNA levels of a proliferation marker than RBC-P. Wettability was the same for both RBCs. Toxicity decreased with increasing number of elution cycles. The initial eluates from RBC-P had a lower toxicity than from RBC-V. SIGNIFICANCE RBCs cured with T2W (delivering both blue and violet light) at higher RE had greater DCs. The greatest DC and the least cell reactions were observed when the RE was >25 J/cm².

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research
04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Kobayashi, Masako; Miron, Richard John; Lussi, Adrian and Schmalz, Gottfried Hans

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0109-5641

Publisher:

Elsevier

Language:

English

Submitter:

Daniela Zesiger

Date Deposited:

02 Dec 2019 16:34

Last Modified:

02 Dec 2019 16:34

Publisher DOI:

10.1016/j.dental.2019.05.015

PubMed ID:

31174864

Uncontrolled Keywords:

Cytotoxicity Light curing unit Resin conversion Resin-based composites

BORIS DOI:

10.7892/boris.135540

URI:

https://boris.unibe.ch/id/eprint/135540

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