Influence of desensitizing and anti-erosive toothpastes on dentine permeability: An in vitro study.

João-Souza, Samira Helena; Scaramucci, Tais; Bühler Borges, Alessandra; Lussi, Adrian; Saads Carvalho, Thiago; Corrêa Aranha, Ana Cecília (2019). Influence of desensitizing and anti-erosive toothpastes on dentine permeability: An in vitro study. Journal of dentistry, 89, p. 103176. Elsevier Science 10.1016/j.jdent.2019.07.014

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This study analyzed the effect of desensitizing and/or anti-erosive toothpastes on dentine permeability.


One-mm dentin discs were prepared from human molars and exposed to EDTA solution (5 min, 17%). Initial dentine permeability was measured, under constant pressure. Specimens were randomly allocated into 10 groups: four anti-erosive toothpastes (calcium silicate + sodium phosphate, potassium nitrate, stannous chloride + chitosan, oligopeptide-104); four desensitizing toothpastes (arginine + calcium carbonate, calcium sodium phosphosilicate, strontium acetate, stannous fluoride); and two controls (regular fluoridated toothpaste, and human saliva). They were submitted to a 5-day erosion-abrasion cycling model. Erosion consisted of immersion in citric acid (2 min, 0.3%, natural pH ˜ 2.6, 4x/day), followed by 1 h exposure to human saliva. Specimens were brushed for 15 s (2 N, 45 strokes) with the toothpaste slurries (total exposure time of 2 min). After 5 cycles, the final dentine permeability was determined. Dentine permeability change was calculated as a percentage of the initial hydraulic conductance (%Lp). Data were analyzed with one-way ANOVA and Tukey tests (α=0.05).


The toothpastes calcium silicate + sodium phosphate and potassium nitrate, showed significant decrease in %Lp, with no difference between them. The regular fluoridated toothpaste also decreased the %Lp, not differing from potassium nitrate. No desensitizing toothpaste showed change in %Lp. Human saliva, oligopeptide-104 and stannous chloride + chitosan presented significant increase in %Lp, without difference between them.


Calcium silicate + sodium phosphate, potassium nitrate, and the regular fluoridated toothpaste decreased dentine permeability, whereas the desensitizing toothpastes tested did not.


Toothpastes had distinct impacts on dentine permeability, which may reflect a variable effect on the treatment of dentine hypersensitivity. Within the limitations of a laboratory-based study, toothpastes with an anti-erosive claim could also be effective in reducing the pain in dentine hypersensitivity.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Niemeyer, Samira Helena; Lussi, Adrian and Saads Carvalho, Thiago


600 Technology > 610 Medicine & health




Elsevier Science




Daniela Zesiger

Date Deposited:

03 Dec 2019 17:13

Last Modified:

07 Jan 2022 08:08

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

Dental abrasion Dental erosion Dentine permeability Hydraulic conductance Toothpastes Tubule occlusion




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