Enamel surface loss after erosive and abrasive cycling with different periods of immersion in human saliva.

Lopes, Raquel Marianna; da Silva, Juliana Sant' Anna; João-Souza, Samira Helena; Maximiano, Vinícius; Machado, Alana Cristina; Scaramucci, Taís; Aranha, Ana Cecília Correa (2020). Enamel surface loss after erosive and abrasive cycling with different periods of immersion in human saliva. Archives of oral biology, 109, p. 104549. Elsevier Science 10.1016/j.archoralbio.2019.104549

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OBJECTIVE This in vitro study aimed to evaluate different periods of exposure to clarified human saliva for the ability to protect enamel against erosive tooth wear. METHODS For this purpose, sixty specimens (4 × 4 × 1.5 mm) were prepared from third human molars. For all groups, the period before abrasion was performed by remineralisation with human saliva (except in G1). The specimens were randomly divided into six groups (n = 10) according to the different remineralisation times of exposure to clarified human saliva: no exposure to saliva (G1) and 30 min (G2), 60 min (G3), 90 min (G4), 120 min (G5), and 240 min (G6) of exposure to human saliva. A 5-day cycling was performed with 5 min of erosion (1% citric acid; pH 2.3), 4x/day. After the first and last erosive episodes, the abrasion challenge was performed with slurry of fluoride toothpaste (1450 ppm F-, as sodium monofluorophosphate) plus human saliva (1:3), with an electric toothbrush (15 s, with a total of 120 s of slurry immersion). Surface loss (SL) was determined using an optical profilometer (n = 10) and for qualitative analysis, environmental scanning electron microscopy (ESEM) was performed (n = 3). The SL data were statistically analysed by one-way analysis of variance (α = 0.05). RESULTS No significant differences were detected among the groups for SL (p > 0.05), and ESEM showed similar aspects of eroded enamel. CONCLUSIONS The period of in vitro exposure to clarified human saliva was not able to protect against enamel erosion.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

João de Souza, Samira Helena


600 Technology > 610 Medicine & health




Elsevier Science




Daniela Zesiger

Date Deposited:

04 Dec 2019 09:29

Last Modified:

08 Dec 2019 02:46

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

Abrasion Enamel Erosion Human saliva Remineralisation





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