Contemporary chiral simulators for capillary zone electrophoresis.

Caslavska, Jitka; Thormann, Wolfgang (2019). Contemporary chiral simulators for capillary zone electrophoresis. (In Press). Electrophoresis Wiley-VCH 10.1002/elps.201900363

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For separation of enantiomers in presence of a chiral selector, data obtained with the 1D dynamic simulators SIMUL5complex and GENTRANS are compared to data predicted by PeakMaster 6, a recently released generalized model of the linear theory of electromigration. Four electrophoretic systems with stereoisomers of weak bases were investigated. They deal with the estimation of input data for complexation together with the elucidation of the origin of observed system peaks, the interference of analyte and system peak migration, the change of enantiomer migration order as function of the selector concentration and the inversion of analyte migration direction in presence of a multiply negatively charged selector. For all systems, data predicted with PeakMaster 6 are in agreement with those of the dynamic simulators and simulation data compare well with experimental data that were monitored with setups featuring conductivity and/or UV absorbance detection along the capillary. SIMUL5complex and GENTRANS provide the full dynamics of any buffer and sample arrangement and require very long execution time intervals. PeakMaster 6 is restricted to conventional CZE, is based on an approximate solution of the transport equations, provides data for realistic experimental conditions within seconds and represents a practical tool for an experimentalist.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Laboratory for Clinical Pharmacology

UniBE Contributor:

Thormann, Wolfgang

ISSN:

0173-0835

Publisher:

Wiley-VCH

Language:

English

Submitter:

Wolfgang Thormann

Date Deposited:

04 Dec 2019 10:44

Last Modified:

04 Dec 2019 10:44

Publisher DOI:

10.1002/elps.201900363

PubMed ID:

31702052

Uncontrolled Keywords:

Capillary electrophoresis Chiral separation Enantiomer Simulation System peak

BORIS DOI:

10.7892/boris.135757

URI:

https://boris.unibe.ch/id/eprint/135757

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