A cytosolic glucosyltransferase is required for conversion of starch to sucrose in Arabidopsis leaves at night

Chia, Tansy; Thorneycroft, David; Chapple, Andrew; Messerli, Gaëlle; Chen, Jychian; Zeeman, Samuel C.; Smith, Steven M.; Smith, Alison M. (2004). A cytosolic glucosyltransferase is required for conversion of starch to sucrose in Arabidopsis leaves at night. The Plant Journal, 37(6), pp. 853-863. 111 RIVER ST, HOBOKEN 07030-5774, NJ USA: Blackwell Science 10.1111/j.1365-313X.2003.02012.x

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Maltose is exported from the Arabidopsis chloroplast as the main product of starch degradation at night. To investigate its fate in the cytosol, we characterised plants with mutations in a gene encoding a putative glucanotransferase (disproportionating enzyme; DPE2), a protein similar to the maltase Q (MalQ) gene product involved in maltose metabolism in bacteria. Use of a DPE2 antiserum revealed that the DPE2 protein is cytosolic. Four independent mutant lines lacked this protein and displayed a decreased capacity for both starch synthesis and starch degradation in leaves. They contained exceptionally high levels of maltose, and elevated levels of glucose, fructose and other malto-oligosaccharides. Sucrose levels were lower than those in wild-type plants, especially at the start of the dark period. A glucosyltransferase activity, capable of transferring one of the glucosyl units of maltose to glycogen or amylopectin and releasing the other, was identified in leaves of wild-type plants. Its activity was sufficient to account for the rate of starch degradation. This activity was absent from dpe2 mutant plants. Based on these results, we suggest that DPE2 is an essential component of the pathway from starch to sucrose and cellular metabolism in leaves at night. Its role is probably to metabolise maltose exported from the chloroplast. We propose a pathway for the conversion of starch to sucrose in an Arabidopsis leaf.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0960-7412

Publisher:

Blackwell Science

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

03 Dec 2019 11:51

Last Modified:

03 Dec 2019 11:51

Publisher DOI:

10.1111/j.1365-313X.2003.02012.x

Uncontrolled Keywords:

Arabidopsis; glucosyltransferase; maltose metabolism; maltose transporter; starch degradation; starch mutants

BORIS DOI:

10.7892/boris.135867

URI:

https://boris.unibe.ch/id/eprint/135867

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