Effect of enamel matrix derivative liquid in combination with a natural bone mineral on new bone formation in a rabbit GBR model.

Kobayashi, Eizaburo; Fujioka-Kobayashi, Masako; Saulacic, Nikola; Schaller, Benoît; Sculean, Anton; Miron, Richard J. (2019). Effect of enamel matrix derivative liquid in combination with a natural bone mineral on new bone formation in a rabbit GBR model. Clinical oral implants research, 30(6), pp. 542-549. Wiley-Blackwell 10.1111/clr.13440

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Recently, a new liquid carrier system for enamel matrix derivate (EMD-liquid) was developed with better physico-chemical properties for improved adsorption of EMD to biomaterial surfaces. The aim of the present study was to investigate the bone regenerative potential of EMD-liquid in combination with a natural bone mineral (NBM) in vivo.


Four 6-mm defects were created in the calvaria of six New Zealand white rabbits. Defects were filled with either (1) control (empty), (2) 20 mg of NBM, or (3) 20 mg of NBM + 20 µl of EMD-liquid (n = 8). All defects were covered with collagen barrier membranes. The bone regenerative potential was investigated by micro-CT and histomorphological analyses at 8 weeks postsurgery.


The mineralized tissue volume was significantly higher in the NBM + EMD-liquid group when compared to control, whereas no difference was observed between the NBM alone and control groups. While no significant difference was observed for horizontal bone defect closure between the NBM + EMD-liquid and the NBM alone groups, NBM + EMD-liquid significantly increased the total mineralized area and reduced the percentage of soft/connective tissue infiltration. No statistically significant difference was observed between the NBM + EMD-liquid group and NBM alone group for amount of mineralized bone.


The addition of EMD-liquid did not lead to statistically significant bone formation when compared to NBM alone. The combination of NBM + EMD-liquid but not NBM alone did however induce superior mineralized tissues when compared to control (empty). Further research investigating the adsorption potential of EMD-liquid to bone-grafting particles with/without collagen may provide valuable insights into future regenerative strategies with enamel matrix proteins.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery

UniBE Contributor:

Kobayashi, Eizaburo, Kobayashi, Masako (B), Saulacic, Nikola, Schaller, Benoît, Sculean, Anton


600 Technology > 610 Medicine & health








Caroline Dominique Zürcher

Date Deposited:

03 Dec 2019 12:56

Last Modified:

29 Mar 2023 23:36

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

EMD GBR Osteogain bone formation enamel matrix derivative guided bone regeneration





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