Rapid evaluation of bioactive Ti-based surfaces using an in vitro titration method.

Zhao, Weitian; Michalik, David; Ferguson, Stephen; Hofstetter, Wily; Lemaître, Jacques; von Rechenberg, Brigitte; Bowen, Paul (2019). Rapid evaluation of bioactive Ti-based surfaces using an in vitro titration method. Nature communications, 10(1), p. 2062. Springer Nature 10.1038/s41467-019-09673-1

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The prediction of implant behavior in vivo by the use of easy-to-perform in vitro methods is of great interest in biomaterials research. Simulated body fluids (SBFs) have been proposed and widely used to evaluate the bone-bonding ability of implant materials. In view of its limitations, we report here a rapid in vitro method based on calcium titration for the evaluation of in vivo bioactivity. Using four different titanium surfaces, this method identifies that alkaline treatment is the key process to confer bioactivity to titanium whereas no significant effect from heat treatment is observed. The presence of bioactive titanium surfaces in the solution during calcium titration induces an earlier nucleation of crystalline calcium phosphates and changes the crystallization pathway. The conclusions from this method are also supported by the standard SBF test (ISO 23317), in vitro cell culture tests using osteoblasts and in vivo animal experiments employing a pelvic sheep model.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Hofstetter, Wilhelm

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2041-1723

Publisher:

Springer Nature

Language:

English

Submitter:

Marla Rittiner

Date Deposited:

23 Dec 2019 14:08

Last Modified:

13 Mar 2021 17:54

Publisher DOI:

10.1038/s41467-019-09673-1

PubMed ID:

31048680

BORIS DOI:

10.7892/boris.136307

URI:

https://boris.unibe.ch/id/eprint/136307

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