Cellular Factor XIII, a Transglutaminase in Human Corneal Keratocytes.

Orosz, Zsuzsanna Z; Bárdos, Helga; Shemirani, Amir H; Beke Debreceni, Ildikó; Lassila, Riitta; Riikonen, Antti S; Kremer Hovinga, Johanna A.; Seiler, Theo G.; van Dorland, Hendrika A.; Schroeder, Verena; Boda, Zoltán; Nemes, László; Früh Epstein, Beatrice; Nagy, Bence; Facskó, Andrea; Kappelmayer, János; Muszbek, László (2019). Cellular Factor XIII, a Transglutaminase in Human Corneal Keratocytes. International journal of molecular sciences, 20(23) Molecular Diversity Preservation International MDPI 10.3390/ijms20235963

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Cellular factor XIII (cFXIII, FXIII-A2), a transglutaminase, has been demonstrated in a few cell types. Its main function is to cross-link proteins by isopeptide bonds. Here, we investigated the presence of cFXIII in cells of human cornea. Tissue sections of the cornea were immunostained for FXIII-A in combination with staining for CD34 antigen or isopeptide cross-links. Isolated corneal keratocytes were also evaluated by immunofluorescent microscopy and flow cytometry. FXIII-A in the corneal stroma was quantified by Western blotting. FXIII-A mRNA was detected by RT-qPCR. The cornea of FXIII-A-deficient patients was evaluated by cornea topography. FXIII-A was detected in 68 ± 13% of CD34+ keratocytes. Their distribution in the corneal stroma was unequal; they were most abundant in the subepithelial tertile. cFXIII was of cytoplasmic localization. In the stroma, 3.64 ng cFXIII/mg protein was measured. The synthesis of cFXIII by keratocytes was confirmed by RT-qPCR. Isopeptide cross-links were detected above, but not within the corneal stroma. Slight abnormality of the cornea was detected in six out of nine FXIII-A-deficient patients. The presence of cFXIII in human keratocytes was established for the first time. cFXIII might be involved in maintaining the stability of the cornea and in the corneal wound healing process.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Haematology and Central Haematological Laboratory
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Hämatologie (Erwachsene)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pavillon 52 > Forschungsgruppe Experimentelle Hämostase

UniBE Contributor:

Kremer Hovinga, Johanna Anna; Seiler, Günter Theodor Michael; van Dorland, Hendrika Anette; Schröder, Verena and Früh Epstein, Beatrice

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1661-6596

Publisher:

Molecular Diversity Preservation International MDPI

Language:

English

Submitter:

Günter Theodor Michael Seiler

Date Deposited:

11 Dec 2019 16:58

Last Modified:

28 Nov 2020 02:30

Publisher DOI:

10.3390/ijms20235963

PubMed ID:

31783511

Uncontrolled Keywords:

cornea factor XIII isopeptide bonds keratocytes transglutaminase

BORIS DOI:

10.7892/boris.136351

URI:

https://boris.unibe.ch/id/eprint/136351

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