Olomski, Florian; Fettelschoss, Victoria; Jonsdottir, Sigridur; Birkmann, Katharina; Thoms, Franziska; Marti, Eliane; Bachmann, Martin F.; Kündig, Thomas M; Fettelschoss-Gabriel, Antonia (2020). Interleukin 31 in insect bite hypersensitivity - Alleviating clinical symptoms by active vaccination against itch. Allergy, 75(4), pp. 862-871. Wiley 10.1111/all.14145
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BACKGROUND
Insect bite hypersensitivity (IBH) is the most common seasonal pruritic allergic dermatitis of horses occurring upon insect bites. In recent years, a major role for IL-31 in allergic pruritus of humans, monkeys, dogs and mice was acknowledged.
OBJECTIVE
Here, we investigate the role of IL-31 in IBH of horses and developed a therapeutic vaccine against equine IL-31 (eIL-31).
METHODS
IL-31 levels were quantified in allergen-stimulated PBMCs and skin punch biopsies of IBH lesions and healthy skin from IBH-affected and healthy horses. The vaccine consisted of eIL-31 covalently coupled to a virus-like particle (VLP) derived from cucumber mosaic virus containing a tetanus toxoid universal T-cell epitope (CuMVTT ). Eighteen IBH-affected horses were recruited and immunized with 300 μg of eIL-31-CuMVTT vaccine or placebo and IBH severity score was recorded.
RESULTS
IL-31 was increased in PBMCs and exclusively detectable in skin lesions of IBH-affected horses. Vaccination against eIL-31 reduced delta clinical scores when compared to previous untreated IBH season of the same horses and to placebo treated horses in the same year. The vaccine was well tolerated without safety concerns throughout the study.
CONCLUSION
TH 2-derived IL-31 is involved in IBH pathology and accordingly the immunotherapeutic vaccination approach targeting IL-31 alleviated clinical scores in affected horses.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology and Immunology 05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research 05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) |
UniBE Contributor: |
Jonsdottir, Sigridur, Marti, Eliane Isabelle, Bachmann, Martin (B) |
Subjects: |
600 Technology > 610 Medicine & health 600 Technology > 630 Agriculture |
ISSN: |
1398-9995 |
Publisher: |
Wiley |
Language: |
English |
Submitter: |
Lee-Anne Brand |
Date Deposited: |
13 Jan 2020 15:36 |
Last Modified: |
29 Mar 2023 23:36 |
Publisher DOI: |
10.1111/all.14145 |
PubMed ID: |
31816097 |
Uncontrolled Keywords: |
Allergic pruritus interleukin 31 vaccination |
BORIS DOI: |
10.7892/boris.137012 |
URI: |
https://boris.unibe.ch/id/eprint/137012 |
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