Interleukin 31 in insect bite hypersensitivity - Alleviating clinical symptoms by active vaccination against itch.

Olomski, Florian; Fettelschoss, Victoria; Jonsdottir, Sigridur; Birkmann, Katharina; Thoms, Franziska; Marti, Eliane; Bachmann, Martin F.; Kündig, Thomas M; Fettelschoss-Gabriel, Antonia (2020). Interleukin 31 in insect bite hypersensitivity - Alleviating clinical symptoms by active vaccination against itch. Allergy, 75(4), pp. 862-871. Wiley 10.1111/all.14145

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BACKGROUND Insect bite hypersensitivity (IBH) is the most common seasonal pruritic allergic dermatitis of horses occurring upon insect bites. In recent years, a major role for IL-31 in allergic pruritus of humans, monkeys, dogs and mice was acknowledged. OBJECTIVE Here, we investigate the role of IL-31 in IBH of horses and developed a therapeutic vaccine against equine IL-31 (eIL-31). METHODS IL-31 levels were quantified in allergen-stimulated PBMCs and skin punch biopsies of IBH lesions and healthy skin from IBH-affected and healthy horses. The vaccine consisted of eIL-31 covalently coupled to a virus-like particle (VLP) derived from cucumber mosaic virus containing a tetanus toxoid universal T-cell epitope (CuMVTT ). Eighteen IBH-affected horses were recruited and immunized with 300 μg of eIL-31-CuMVTT vaccine or placebo and IBH severity score was recorded. RESULTS IL-31 was increased in PBMCs and exclusively detectable in skin lesions of IBH-affected horses. Vaccination against eIL-31 reduced delta clinical scores when compared to previous untreated IBH season of the same horses and to placebo treated horses in the same year. The vaccine was well tolerated without safety concerns throughout the study. CONCLUSION TH 2-derived IL-31 is involved in IBH pathology and accordingly the immunotherapeutic vaccination approach targeting IL-31 alleviated clinical scores in affected horses.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology, Clinical Immunology and Allergology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Jonsdottir, Sigridur; Marti, Eliane Isabelle and Bachmann, Martin

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

1398-9995

Publisher:

Wiley

Language:

English

Submitter:

Lee-Anne Brand

Date Deposited:

13 Jan 2020 15:36

Last Modified:

23 Apr 2020 01:32

Publisher DOI:

10.1111/all.14145

PubMed ID:

31816097

Uncontrolled Keywords:

Allergic pruritus interleukin 31 vaccination

BORIS DOI:

10.7892/boris.137012

URI:

https://boris.unibe.ch/id/eprint/137012

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