Antibiotic and quaternary ammonium compound resistance in Escherichia coli from calves at the beginning of the -fattening period in Switzerland (2017).

Hausherr, Anna; Becker, Jens Michael; Meylan, Mireille; Wüthrich, Dominik; Collaud, Alexandra; Rossano, Alexandra; Perreten, Vincent (2019). Antibiotic and quaternary ammonium compound resistance in Escherichia coli from calves at the beginning of the -fattening period in Switzerland (2017). Schweizer Archiv für Tierheilkunde, 161(11), pp. 741-748. Gesellschaft Schweizer Tierärztinnen und Tierärzte 10.17236/sat00233

[img] Text
SAT_11_2019_Perreten.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (372kB) | Request a copy

INTRODUCTION In the Swiss veal calf production, antimicrobials and disinfectants are used to control bacterial infectious diseases, leading to a risk of selecting for a resistant bacterial population. While the prevalence of antibiotic resistance in E. coli from calves has been monitored at slaughterhouses in Switzerland since 2006, the resistance situation of E. coli from young calves entering the fattening period is not known. A total of 100 calves entering the fattening period in 20 geographically distant farms in Switzerland were screened for the presence of E. coli using rectal swabs in 2017. Genetic diversity between isolates was determined using repetitive palindromic Polymerase Chain Reaction (rep-PCR) revealing a genetically diverse E. coli population. Susceptibility to 13 antibiotics and to alkyldimethylbenzylammonium (ADBAC) was determined by the measurement of the minimal inhibitory concentration. Antibiotic and quaternary ammonium compound (QAC) resistance genes were identified using microarray and Polymerase Chain Reaction (PCR). Sixty-four percent of the isolates were resistant to at least one antibiotic, and 52% also exhibited decreased susceptibility to ADBAC. Resistance to more than 3 antibiotics was found in 40% of the isolates. Isolates exhibited resistance to tetracycline (57%) associated with the presence of tet genes (tet(A), (B), (E), (G)), to sulfonamides (61%) (sul1, sul2, sul3), ampicillin (56%) (blaTEM-1), trimethoprim (32%) (dfrA), phenicols (31%) (catA1, cmlA1, floR), gentamicin (27%) (ant(2")-Ia, aac(3)-IVa, aac(3)-VIa), and cefotaxime (2%) (blaCTX-M-14 (ESBL)). Mutations in GyrA (S83L) and ParC (S80I) were found in the fluoroquinolone-resistant isolates (6%). All isolates were susceptible to colistin, tigecycline and meropenem. No association between the presence of decreased susceptibility to ADBAC and qac genes was observed. In conclusion, antibiotic and QAC resistant E. coli are present in the gastrointestinal tract of young calves at the beginning of the fattening period, emphasizing the need for appropriate and reduced use of antibiotics and QAC-containing disinfectants in order to limit further selection of these bacteria during the fattening period.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology > Molecular Bacterial Epidemiology and Infectiology
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Hausherr, Anna; Becker, Jens Michael; Meylan, Mireille; Wüthrich, Dominik; Collaud, Alexandra; Rossano, Alexandra and Perreten, Vincent

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

1664-2848

Publisher:

Gesellschaft Schweizer Tierärztinnen und Tierärzte

Language:

English

Submitter:

Vincent Perreten

Date Deposited:

13 Jan 2020 15:31

Last Modified:

15 Jan 2020 14:28

Publisher DOI:

10.17236/sat00233

PubMed ID:

31685447

Uncontrolled Keywords:

Antibiotic; cattle; disinfectants; Enterobacteriaceae; resistance; QAC Antibiotico; bovini; disinfettanti; enterobatteriacee; resistenza; QAC Antibiotika; Desinfektionsmittel; Enterobacteriaceae; Resistenz; Rind; QAC antibiotique; bovins; désinfectants; enterobacteriacées; résistance; QAC

BORIS DOI:

10.7892/boris.137015

URI:

https://boris.unibe.ch/id/eprint/137015

Actions (login required)

Edit item Edit item
Provide Feedback