Tytgat, Hanne L P; Lin, Chia-Wei; Levasseur, Mikail D; Tomek, Markus B; Rutschmann, Christoph; Mock, Jacqueline; Liebscher, Nora; Terasaka, Naohiro; Azuma, Yusuke; Wetter, Michael; Bachmann, Martin F.; Hilvert, Donald; Aebi, Markus; Keys, Timothy G (2019). Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assemblies. Nature communications, 10(1), p. 5403. Springer Nature 10.1038/s41467-019-13283-2
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Glycosylation of proteins profoundly impacts their physical and biological properties. Yet our ability to engineer novel glycoprotein structures remains limited. Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively. Here, we circumvent these bottlenecks by developing a facile glycoengineering platform that operates in the bacterial cytoplasm. The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm. We exploit the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope. This work establishes cytoplasmic glycoengineering as a powerful platform for producing glycoprotein structures with diverse future biomedical applications.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology and Immunology |
UniBE Contributor: |
Bachmann, Martin (B) |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2041-1723 |
Publisher: |
Springer Nature |
Language: |
English |
Submitter: |
Lee-Anne Brand |
Date Deposited: |
13 Jan 2020 16:00 |
Last Modified: |
29 Mar 2023 23:36 |
Publisher DOI: |
10.1038/s41467-019-13283-2 |
PubMed ID: |
31776333 |
BORIS DOI: |
10.7892/boris.137017 |
URI: |
https://boris.unibe.ch/id/eprint/137017 |
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