Translational offsetting as a mode of estrogen receptor α‐dependent regulation of gene expression

Lorent, Julie; Kusnadi, Eric P; Hoef, Vincent; Rebello, Richard J; Leibovitch, Matthew; Ristau, Johannes; Chen, Shan; Lawrence, Mitchell G; Szkop, Krzysztof J; Samreen, Baila; Balanathan, Preetika; Rapino, Francesca; Close, Pierre; Bukczynska, Patricia; Scharmann, Karin; Takizawa, Itsuhiro; Risbridger, Gail P; Selth, Luke A; Leidel, Sebastian A; Lin, Qishan; ... (2019). Translational offsetting as a mode of estrogen receptor α‐dependent regulation of gene expression. The EMBO journal, 38(23), e101323. EMBO Press 10.15252/embj.2018101323

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Estrogen receptor alpha (ERα) activity is associated with increased cancer cell proliferation. Studies aiming to understand the impact of ERα on cancer-associated phenotypes have largely been limited to its transcriptional activity. Herein, we demonstrate that ERα coordinates its transcriptional output with selective modulation of mRNA translation. Importantly, translational perturbations caused by depletion of ERα largely manifest as "translational offsetting" of the transcriptome, whereby amounts of translated mRNAs and corresponding protein levels are maintained constant despite changes in mRNA abundance. Transcripts whose levels, but not polysome association, are reduced following ERα depletion lack features which limit translation efficiency including structured 5'UTRs and miRNA target sites. In contrast, mRNAs induced upon ERα depletion whose polysome association remains unaltered are enriched in codons requiring U34-modified tRNAs for efficient decoding. Consistently, ERα regulates levels of U34-modifying enzymes and thereby controls levels of U34-modified tRNAs. These findings unravel a hitherto unprecedented mechanism of ERα-dependent orchestration of transcriptional and translational programs that may be a pervasive mechanism of proteome maintenance in hormone-dependent cancers.

Item Type:

Journal Article (Original Article)


08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Leidel, Sebastian Andreas


500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry




EMBO Press




Christina Schüpbach

Date Deposited:

13 Jan 2020 08:53

Last Modified:

28 Nov 2020 02:30

Publisher DOI:


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