Antibiotic Susceptibility Patterns of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis Strains from Different Decades.

Kulik, Eva M; Thurnheer, Thomas; Karygianni, Lamprini; Walter, Clemens; Sculean, Anton; Eick, Sigrun (2019). Antibiotic Susceptibility Patterns of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis Strains from Different Decades. Antibiotics, 8(4) MDPI 10.3390/antibiotics8040253

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The aim of this study was to determine the antibiotic susceptibility patterns of 57 Aggregatibacter actinomycetemcomitans and 56 Porphyromonas gingivalis strains isolated from subgingival biofilm samples of periodontitis patients in Switzerland from 1980 to 2017. The minimal inhibitory concentrations (MIC) of the most commonly used antibiotics in periodontal therapy (amoxicillin, metronidazole, azithromycin, and doxycycline) or in severe body infections (amoxicillin/clavulanic acid, clindamycin, ertapenem, and moxifloxacin) were determined. Furthermore, all the strains were screened for beta-lactamase activity and the presence of selected resistance genes (cfxA, ermF, and tetQ). Overall, there was no significant increase in MIC values over the 37‑year period. Two of the most recent P. gingivalis isolates yielded the highest MIC values. The first isolate was ermF-positive with MIC values >8 µg/mL, 2 µg/mL, and 0.25 µg/mL for clindamycin, azithromycin, and moxifloxacin, respectively. The second isolate showed a high MIC value of 4 µg/mL for moxifloxacin, which was associated with a confirmed single-point mutation in the quinolone resistance-determining region (QRDR) of the gyrA gene. Although there was no significant increase in the antibiotic resistance among the oral bacterial isolates tested, the detection of resistant P. gingivalis isolates underlines the need to optimize the antibiotic therapeutic protocols in dentistry.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Sculean, Anton and Eick, Sigrun

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2079-6382

Publisher:

MDPI

Language:

English

Submitter:

Doris Burri

Date Deposited:

14 Jan 2020 14:51

Last Modified:

28 Nov 2020 02:30

Publisher DOI:

10.3390/antibiotics8040253

PubMed ID:

31817588

Uncontrolled Keywords:

beta lactams minimal inhibitory concentrations moxifloxacin periodontal pathogens

BORIS DOI:

10.7892/boris.137262

URI:

https://boris.unibe.ch/id/eprint/137262

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