Effects of argon plasma treatment on the osteoconductivity of bone grafting materials.

Canullo, Luigi; Genova, Tullio; Rakic, Mia; Sculean, Anton; Miron, Richard; Muzzi, Maurizio; Carossa, Stefano; Mussano, Federico (2020). Effects of argon plasma treatment on the osteoconductivity of bone grafting materials. Clinical oral investigations, 24(8), pp. 2611-2623. Springer-Verlag 10.1007/s00784-019-03119-0

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BACKGROUND The osteoconductive properties of bone grafting materials represent one area of research for the management of bony defects found in the fields of periodontology and oral surgery. From a physico-chemical aspect, the wettability of the graft has been demonstrated to be one of the most important factors for new bone formation. It is also well-known that argon plasma treatment (PAT) and ultraviolet irradiation (UV) may increase the surface wettability and, consequently, improve the regenerative potential of the bone grafts. Therefore, the aim of the present in vitro study was to evaluate the effect of PAT and UV treatment on the osteoconductive potential of various bone grafts. MATERIALS AND METHODS The following four frequently used bone grafts were selected for this study: synthetic hydroxyapatite (Mg-HA), biphasic calcium phosphate (BCP), cancellous and cortical xenogenic bone matrices (CaBM, CoBM). Sixty-six serially numbered disks 10 mm in diameter were used for each graft material and randomly assigned to the following three groups: test 1 (PAT), test 2 (UV), and control (no treatment). Six samples underwent topographic analysis using SEM pre- and post-treatments to evaluate changes in surface topography/characteristics. Additionally, cell adhesion and cell proliferation were evaluated at 2 and 72 h respectively following incubation in a three-dimensional culture system utilizing a bioreactor. Furthermore, the effects of PAT and UV on immune cells were assessed by measuring the viability of human macrophages at 24 h. RESULTS The topographic analysis showed different initial morphologies of the commercial biomaterials (e.g., Mg-HA and BCP showed flat morphology; BM samples were extremely porous with high roughness). The surface analysis following experimental treatments did not demonstrate topographical difference when compared with controls. Investigation of cells demonstrated that PAT treatment significantly increased cell adhesion of all 4 evaluated bone substitutes, whereas UV failed to show any statistically significant differences. The viability test revealed no differences in terms of macrophage adhesion on any of the tested surfaces. CONCLUSION Within their limitations, the present results suggest that treatment of various bone grafting materials with PAT appears to enhance the osteoconductivity of bone substitutes in the early stage by improving osteoblast adhesion without concomitantly affecting macrophage viability. CLINICAL RELEVANCE Treatment of bone grafts with PAT appears to result in faster osseointegration of the bone grafting materials and may thus favorably influence bone regeneration.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Sculean, Anton and Miron, Richard John

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1432-6981

Publisher:

Springer-Verlag

Language:

English

Submitter:

Doris Burri

Date Deposited:

17 Jan 2020 13:52

Last Modified:

16 Jul 2020 01:31

Publisher DOI:

10.1007/s00784-019-03119-0

PubMed ID:

31748982

Uncontrolled Keywords:

Bio-activation Bone graft Osseointegration Osteoconductivity Plasma of argon

BORIS DOI:

10.7892/boris.137278

URI:

https://boris.unibe.ch/id/eprint/137278

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