Clinical evaluation of a newly developed chairside test to determine periodontal pathogens.

Arweiler, Nicole B.; Marx, Vanessa K.; Laugisch, Oliver; Sculean, Anton; Auschill, Thorsten M (2020). Clinical evaluation of a newly developed chairside test to determine periodontal pathogens. Journal of periodontology, 91(3), pp. 387-395. American Academy of Periodontology 10.1002/JPER.19-0180

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BACKGROUND The subgingival microbiota as well as determination of markers such as associated pathogens is still in the focus of dental research. The aim of this controlled clinical trial was to determine clinical applicability of a newly developed chairside bacterial test (CST) for the most relevant periodontal pathogens. METHODS Within 125 participants (100 with periodontitis, 25 healthy) two sulcus fluid samples each were collected and pooled for further analysis. Samples were analyzed with CST and results (positive signals for every pathogen/control) were visually detected by eye. As a reference quantitative polymerase chain reaction (qPCR) was performed. RESULTS The detection limit of CST revealed 1.2 × 104 for Treponema denticola (T.d.) and Tannerella forsythia (T.f.), 2.5 × 104 for Porphyromonas gingivalis (P.g.), 5.3 × 103 for Prevotella intermedia (P.i.), and 5.8 × 104 for Aggregatibacter actinomycetemcomitans (A.a.). Based on this maximum potential of positive detections, the sensitivities of CST in reference to qPCR were: T.d. (91.3%); T.f. (86.3%); P.g. (83.8%); P.i. (85.7%), and A.a. (100%). In regard to the clinical diagnosis, the CST assay and the qPCR method reached a sensitivity of 87.82% and 94%, respectively. The specificity for both methods was 100%. CONCLUSION This newly developed CST can detect five typical periodontal pathogens with a somewhat lower sensitivity towards qPCR that can be classified as "good."

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Laugisch, Oliver and Sculean, Anton

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0022-3492

Publisher:

American Academy of Periodontology

Language:

English

Submitter:

Doris Burri

Date Deposited:

20 Jan 2020 11:22

Last Modified:

22 Mar 2020 01:32

Publisher DOI:

10.1002/JPER.19-0180

PubMed ID:

31393600

Uncontrolled Keywords:

PCR diagnosis microbiology periodontal disease periodontitis point-of-care

BORIS DOI:

10.7892/boris.137322

URI:

https://boris.unibe.ch/id/eprint/137322

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