Gingipains impair attachment of epithelial cell to dental titanium abutment surfaces.

Eick, Sigrun; Gadzo, Naida; Tacchi, Manuel; Sculean, Anton; Potempa, Jan; Stavropoulos, Andreas (2019). Gingipains impair attachment of epithelial cell to dental titanium abutment surfaces. Journal of biomedical materials research. Part B - applied biomaterials, 107(8), pp. 2549-2556. John Wiley & Sons 10.1002/jbm.b.34345

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The study investigated in vitro the effect of Porphyromonas gingivalis and its cysteine proteases (gingipains) on epithelial cell adhesion to titanium-zirconium alloy surfaces. Titanium-zirconium discs with a standard machined (M) or chemically modified hydrophilic surface (modM) were coated with lamin-5 and incubated with telomerase-inactivated gingival keratinocytes (TIGK). Three P. gingivalis strains or gingipains were either added simultaneously with TIGK or after TIGK cells were already attached to the disks. Adhered TIGK cells were counted at 24 h. All P. gingivalis strains clearly inhibited adhesion of TIGK cells to M and modM surfaces. Compared with bacteria/gingipain-free TIGK cell cultures, the number of attached TIGK cells was reduced by about 80% and 60% when P. gingivalis was added simultaneously or after TIGK cells were already attached to the disks (each p < 0.01), respectively. Counts of attached cells were similarly reduced when only gingipains were used. Adhesion molecules of TIGK cells, in particular E-cadherin, were cleaved by P. gingivalis. In conclusion, P. gingivalis and gingipains interfere with the adhesion of epithelial cells to titanium-zirconium alloy surfaces by cleaving adhesion molecules, while a chemically modified hydrophilic titanium-zirconium alloy surface did not yield any protection. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2549-2556, 2019.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > Department of Orthodontics
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Eick, Sigrun; Tacchi, Manuel and Sculean, Anton

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1552-4973

Publisher:

John Wiley & Sons

Language:

English

Submitter:

Renate Imhof-Etter

Date Deposited:

06 Jan 2020 16:07

Last Modified:

01 Dec 2020 02:30

Publisher DOI:

10.1002/jbm.b.34345

PubMed ID:

30763463

Uncontrolled Keywords:

Porphyromonas gingivalis dental implants epithelial cells titanium

BORIS DOI:

10.7892/boris.137355

URI:

https://boris.unibe.ch/id/eprint/137355

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