BMP7 aberrantly induced in the psoriatic epidermis instructs inflammation-associated Langerhans cells.

Borek, Izabela; Köffel, René; Feichtinger, Julia; Spies, Melanie; Glitzner-Zeis, Elisabeth; Hochgerner, Mathias; Sconocchia, Tommaso; Krump, Corinna; Tam-Amersdorfer, Carmen; Passeger, Christina; Benezeder, Theresa; Tittes, Julia; Redl, Anna; Painsi, Clemens; Thallinger, Gerhard G; Wolf, Peter; Stary, Georg; Sibilia, Maria; Strobl, Herbert (2020). BMP7 aberrantly induced in the psoriatic epidermis instructs inflammation-associated Langerhans cells. Journal of allergy and clinical immunology, 145(4), 1194-1207.e11. Elsevier 10.1016/j.jaci.2019.12.011

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BACKGROUND Epidermal hyperplasia represents a morphologic hallmark of psoriatic skin lesions. Langerhans cells (LCs) in the psoriatic epidermis engage with keratinocytes (KCs) in tight physical interactions; moreover, they induce T cell-mediated immune responses critical to psoriasis. OBJECTIVE Epidermal factors in psoriasis pathogenesis remain poorly understood. METHODS We phenotypically characterized BMP7-LCs vs. TGF-β1-LCs and analyzed their functional properties using flow cytometry, cell kinetic studies, co-culture with CD4 T-cells and cytokine measurements. Furthermore, immunohistology of healthy and psoriatic skin was performed. Additionally, in vivo experiments with Junf/fJunBf/fK5creER mice were carried out to assess the role of BMP signaling in psoriatic skin inflammation. RESULTS Here we identified a KC-derived signal, i.e. bone morphogenetic protein (BMP) signaling, to promote epidermal changes in psoriasis. Whereas BMP7 is strictly confined to the basal KC layer in the healthy skin, it is expressed at high levels throughout the lesional psoriatic epidermis. BMP7 instructs precursor cells to differentiate into LCs that phenotypically resemble psoriatic LCs. These BMP7-LCs exhibit proliferative activity and increased sensitivity to bacterial stimulation. Moreover, aberrant high BMP signaling in the lesional epidermis is mediated by a KC intrinsic mechanism, as suggested from murine data and clinical outcome after topical anti-psoriatic treatment in human patients. CONCLUSION Our data indicate that available TGF-β family members within the lesional psoriatic epidermis preferentially signal through the canonical BMP signaling cascade to instruct inflammatory-type LCs and to promote psoriatic epidermal changes. Targeting BMP signaling might allow to therapeutically interfere with cutaneous psoriatic manifestations.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Cell Biology

UniBE Contributor:

Köffel, René

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1097-6825

Publisher:

Elsevier

Language:

English

Submitter:

René Köffel

Date Deposited:

08 Jan 2020 11:29

Last Modified:

12 Apr 2020 01:32

Publisher DOI:

10.1016/j.jaci.2019.12.011

PubMed ID:

31870764

Uncontrolled Keywords:

BMP7 Langerhans cells Psoriasis TGF-β signaling

BORIS DOI:

10.7892/boris.137634

URI:

https://boris.unibe.ch/id/eprint/137634

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