DBBM shows no signs of resorption under inflammatory conditions. An experimental study in the mouse calvaria.

Kuchler, Ulrike; Dos Santos, Gabriel Mulinari; Heimel, Patrick; Stähli, Alexandra; Strauss, Franz Josef; Tangl, Stefan; Gruber, Reinhard (2020). DBBM shows no signs of resorption under inflammatory conditions. An experimental study in the mouse calvaria. Clinical oral implants research, 31(1), pp. 10-17. Wiley-Blackwell 10.1111/clr.13538

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OBJECTIVES Deproteinized bovine bone mineral (DBBM) is not resorbable. However, the behavior of DBBM under inflammatory conditions remains unclear. Aim of the study was therefore to evaluate the resorption of DBBM under local inflammatory conditions in vivo using the calvarial osteolysis model. METHODS In thirty adult BALB/c mice, DBBM was implanted into the space between the elevated soft tissue and the calvarial bone. Inflammation was induced either by lipopolysaccharides (LPS) injection or by polyethylene particles (Ceridust) mixed with DBBM. Three modalities were randomly applied (n = 10 each): (a) DBBM alone (control), (b) DBBM + LPS, and (c) DBBM + polyethylene particles (Ceridust). Mice were euthanized on day fourteen, and each calvarium was subjected to histological and µCT analysis. Primary outcome was the size distribution of the DBBM particles. Secondary outcome was the surface erosion of the calvarial bone. RESULTS Histological and µCT analysis revealed that the size distribution and the volume of DBBM particles in the augmented site were similar between DBBM alone and the combinations with LPS or polyethylene particles. Moreover, histological evaluation showed no signs of erosions of DBBM particles under inflammatory conditions. µCT analysis and histology further revealed that LPS and the polyethylene particles, but not the DBBM alone, caused severe erosions of the calvarial bone as indicated by large voids representing the massive compensatory new immature woven bone formation on the endosteal surface. CONCLUSIONS Local calvarial bone but not the DBBM particles undergo severe resorption and subsequent new bone formation under inflammatory conditions in a mouse model.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Stähli, Alexandra Beatrice and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0905-7161

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Doris Burri

Date Deposited:

16 Jan 2020 08:49

Last Modified:

16 Jan 2020 08:49

Publisher DOI:

10.1111/clr.13538

PubMed ID:

31529644

Uncontrolled Keywords:

bone regeneration bone substitutes calvaria inflammation mice osteoclasts

BORIS DOI:

10.7892/boris.137785

URI:

https://boris.unibe.ch/id/eprint/137785

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