Single cell transcriptome atlas of the Drosophila larval brain.

Brunet Avalos, Clarisse; Maier, G Larisa; Bruggmann, Rémy; Sprecher, Simon G (2019). Single cell transcriptome atlas of the Drosophila larval brain. eLife, 8 eLife Sciences Publications 10.7554/eLife.50354

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Cell diversity of the brain and how it is affected by starvation, remains largely unknown. Here, we introduce a single cell transcriptome atlas of the entire Drosophila first instar larval brain. We first assigned cell-type identity based on known marker genes, distinguishing five major groups: neural progenitors, differentiated neurons, glia, undifferentiated neurons and non-neural cells. All major classes were further subdivided into multiple subtypes, revealing biological features of various cell-types. We further assessed transcriptional changes in response to starvation at the single-cell level. While after starvation the composition of the brain remains unaffected, transcriptional profile of several cell clusters changed. Intriguingly, different cell-types show very distinct responses to starvation, suggesting the presence of cell-specific programs for nutrition availability. Establishing a single-cell transcriptome atlas of the larval brain provides a powerful tool to explore cell diversity and assess genetic profiles from developmental, functional and behavioral perspectives.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Bioinformatics and Computational Biology > Bioinformatics
08 Faculty of Science > Department of Biology > Bioinformatics and Computational Biology > Computational Biology
08 Faculty of Science > Department of Biology > Bioinformatics and Computational Biology

UniBE Contributor:

Bruggmann, Rémy

ISSN:

2050-084X

Publisher:

eLife Sciences Publications

Language:

English

Submitter:

Andrea Stettler

Date Deposited:

30 Dec 2019 16:08

Last Modified:

04 May 2023 12:49

Publisher DOI:

10.7554/eLife.50354

PubMed ID:

31746739

Uncontrolled Keywords:

D. melanogaster Drosophila brain cell diversity larva nervous system neuroscience single cell transcriptome

BORIS DOI:

10.7892/boris.137794

URI:

https://boris.unibe.ch/id/eprint/137794

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