Biologization of Collagen-Based Biomaterials Using Liquid-Platelet-Rich Fibrin: New Insights into Clinically Applicable Tissue Engineering.

Al-Maawi, Sarah; Herrera-Vizcaíno, Carlos; Orlowska, Anna; Willershausen, Ines; Sader, Robert; Miron, Richard J.; Choukroun, Joseph; Ghanaati, Shahram (2019). Biologization of Collagen-Based Biomaterials Using Liquid-Platelet-Rich Fibrin: New Insights into Clinically Applicable Tissue Engineering. Materials, 12(23) Molecular Diversity Preservation International MDPI 10.3390/ma12233993

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Platelet-rich fibrin (PRF) is a blood concentrate derived from venous blood that is processed without anticoagulants by a one-step centrifugation process. This three-dimensional scaffold contains inflammatory cells and plasma proteins entrapped in a fibrin matrix. Liquid-PRF was developed based on the previously described low-speed centrifuge concept (LSCC), which allowed the introduction of a liquid-PRF formulation of fibrinogen and thrombin prior to its conversion to fibrin. Liquid-PRF was introduced to meet the clinical demand for combination with biomaterials in a clinically applicable and easy-to-use way. The aim of the present study was to evaluate, ex vivo, the interaction of the liquid-PRF constituents with five different collagen biomaterials by histological analyses. The results first demonstrated that large variability existed between the biomaterials investigated. Liquid-PRF was able to completely invade Mucograft® (MG; Geistlich Biomaterials, Wolhusen, Switzerland) and to partly invade Bio-Gide® (BG; Geistlich Biomaterials, Wolhusen, Switzerland) and Mucoderm® (MD; Botiss Biomaterials, Berlin, Germany), and Collprotect® (CP; Botiss Biomaterials, Berlin, Germany) showed only a superficial interaction. The BEGO® collagen membrane (BCM; BEGO Implant Systems) appeared to be completely free of liquid-PRF. These results were confirmed by the different cellular penetration and liquid-PRF absorption coefficient (PAC) values of the evaluated membranes. The present study demonstrates a system for loading biomaterials with a complex autologous cell system (liquid-PRF) in a relatively short period of time and in a clinically relevant manner. The combination of biomaterials with liquid-PRF may be clinically utilized to enhance the bioactivity of collagen-based biomaterials and may act as a biomaterial-based growth factor delivery system.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Miron, Richard John

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1996-1944

Publisher:

Molecular Diversity Preservation International MDPI

Language:

English

Submitter:

Doris Burri

Date Deposited:

20 Jan 2020 14:13

Last Modified:

20 Jan 2020 14:13

Publisher DOI:

10.3390/ma12233993

PubMed ID:

31810182

Uncontrolled Keywords:

LSCC centrifugation collagen leukocytes liquid-PRF platelet-rich fibrin platelets tissue engineering

BORIS DOI:

10.7892/boris.137810

URI:

https://boris.unibe.ch/id/eprint/137810

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