Fluid platelet-rich fibrin stimulates greater dermal skin fibroblast cell migration, proliferation, and collagen synthesis when compared to platelet-rich plasma.

Wang, Xuzhu; Yang, Yang; Zhang, Yufeng; Miron, Richard J. (2019). Fluid platelet-rich fibrin stimulates greater dermal skin fibroblast cell migration, proliferation, and collagen synthesis when compared to platelet-rich plasma. Journal of cosmetic dermatology, 18(6), pp. 2004-2010. Wiley 10.1111/jocd.12955

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BACKGROUND Regenerative therapies in the field of facial aesthetics have become a growing field of interest with many recent advancements made over the past decade to meet the growing worldwide demand. While first versions of platelet-derived concentrates were formulated with anticoagulants (PRP), recent modifications to centrifugation speeds and times have permitted the development of a liquid platelet-rich fibrin (fluid-PRF) without use of anticoagulants. OBJECTIVE To compare this entirely natural platelet concentrate (fluid-PRF) to formally utilized PRP on skin cell behavior and regeneration. METHODS Dermal skin fibroblast was cultivated with either fluid-PRF or PRP and investigated for their ability to promote/influence cell viability, migration, spreading, proliferation, and mRNA levels of known mediators of dermal biology including PDGF, TGF-beta, and fibronectin. RESULTS All platelet concentrates were nontoxic to cells demonstrating high cell survival. Skin fibroblasts migrated over 350% more in fluid-PRF when compared to control and PRP (200% increase). Fluid-PRF also significantly induced greater cell proliferation at 5 days. While both PRP and fluid-PRF induced significantly elevated cell mRNA levels of PDGF, it was observed that TGF-beta, collagen 1, and fibronectin mRNA levels were all significantly highest in the fluid-PRF group. Lastly, fluid-PRF demonstrated a significantly greater ability to induce collagen matrix synthesis when compared to PRP. CONCLUSION The findings from the present study demonstrate greater regenerative potential of fluid-PRF on human skin fibroblasts. Future clinical use of fluid-PRF in the field of facial aesthetics is necessary to further evaluate the potential advantages of anticoagulant removal from platelet concentrates.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Miron, Richard John

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1473-2130

Publisher:

Wiley

Language:

English

Submitter:

Doris Burri

Date Deposited:

20 Jan 2020 15:44

Last Modified:

20 Jan 2020 15:44

Publisher DOI:

10.1111/jocd.12955

PubMed ID:

30990574

Uncontrolled Keywords:

PRF facial aesthetics i-PRF injectable PRF liquid PRF platelet-rich fibrin skin rejuvenation

BORIS DOI:

10.7892/boris.137817

URI:

https://boris.unibe.ch/id/eprint/137817

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