Growth Factor Delivery to a Bovine Defect Using Leukocyte -Rich Platelet-Rich Concentrates on a Hyaluronic Acid Scaffold.

Titan, Ashley; Schär, Michael; Hutchinson, Ian; Demange, Marco; Chen, Tony; Rodeo, Scott (2020). Growth Factor Delivery to a Bovine Defect Using Leukocyte -Rich Platelet-Rich Concentrates on a Hyaluronic Acid Scaffold. Arthroscopy - the journal of arthroscopic & related surgery, 36(5), pp. 1431-1440. Elsevier 10.1016/j.arthro.2019.12.004

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To determine if (1) Human L-PRP or (2) L-PRF delivered on a HA scaffold at a bovine chondral defect, a simulated cartilage tear interface, in vitro would improve tissue formation based on biomechanical, histological, and biochemical measures.


L-PRF and L-PRP was prepared from 3 healthy volunteer donors which was delivered in conjunction with Hyaluronic acid (HA scaffolds) to defects created in full thickness bovine cartilage plugs harvested from bovine femoral condyle and trochlea. Specimens were cultured in vitro for up to 42 days. Treatment groups included an HA scaffold alone and scaffolds containing L-PRF or L-PRP. Cartilage repair was assessed using biomechanical testing, histology, DNA quantification, and measurement of sulfated glycosaminoglycan (sGAG) and collagen content at 28 and 42 days.


L-PRF elicited the greatest degree of defect filling and improvement in other histological measures. L-PRF treated specimens also had the greatest cellularity when compared to L-PRP and control at day 28 (560.4 vs. 191.4 vs. 124.2, p=00.15); at day 48 there remains a difference though not significant between L-PRF vs L-PRP, (761.1 vs 589.3, p=0.219) . L-PRF had greater collagen deposition when compared to L-PRP at day 42 (40.1 vs 16.3, p< 0.0001). L-PRF had significantly higher maximum interfacial strength compared to the control at day 42 (10.92 N vs 0.66 N, p=0.015), but had no significant difference compared to L-PRP (10.92 N vs 6.58, p=0.536). L-PRP facilitated a greater amount of sGAG production at day 42 when compared to L-PRF (15.9 vs. 4.3, p=0.009).


Delivery of leukocyte rich platelet concentrates in conjunction with a HA scaffold may allow for improvements in cartilage healing through different pathways. L-PRF was not superior to L-PRP in its biomechanical strength suggesting that both treatments may be effective in improving biomechanical strength of healing cartilage through different pathways.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery

UniBE Contributor:

Schär, Michael


600 Technology > 610 Medicine & health








Kathrin Aeschlimann

Date Deposited:

07 Jan 2020 14:49

Last Modified:

05 Dec 2022 15:35

Publisher DOI:


PubMed ID:


Additional Information:

Ashley Titan, Michael Schär: equal contribution of the two authors

Uncontrolled Keywords:

PRP cartilage repair leukocyte rich platelet enriched concentrates leukocyte-rich platelet-rich fibrin




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