Analysis of fragmented oxidized phosphatidylcholines in human plasma using mass spectrometry: Comparison with immune assays.

Philippova, Maria; Oskolkova, Olga V; Bicker, Wolfgang; Schoenenberger, Andreas W.; Resink, Therese J; Erne, Paul; Bochkov, Valery N (2019). Analysis of fragmented oxidized phosphatidylcholines in human plasma using mass spectrometry: Comparison with immune assays. Free radical biology & medicine, 144, pp. 167-175. Elsevier 10.1016/j.freeradbiomed.2019.05.027

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Circulating oxidized phospholipids are increasingly recognized as biomarkers of atherosclerosis. Clinical association studies have been mainly performed using an immune assay based on monoclonal antibody E06, which recognizes a variety of molecular species of oxidized phosphatidylcholine (OxPC) in lipoproteins, cell membranes or covalently bound to plasma proteins. Accumulating evidence shows that individual molecular species of OxPC demonstrate different biological activities and have different half-life times. Therefore, it is likely that certain molecular species can be associated with pathology more strongly than others. This hypothesis can only be tested using LC-MS/MS allowing quantification of individual molecular species of OxPCs. In order to ensure that laborious LC-MS/MS methods do not simply replicate the results of a technically simpler E06-OxPCs assay, we have performed relative quantification of 8 truncated molecular species of OxPCs in plasma of 132 probands and compared the data with the results of the E06-OxPCs and OxLDL assays. We have found a strong correlation between individual molecular species of OxPCs but only a weak correlation of LC-MS/MS-OxPCs data with the E06-OxPCs assay and no correlation with the OxLDL assay. Furthermore, in contrast to the results of E06-OxPCs or OxLDL assays, 7 out of 8 OxPC species were associated with hypertension. The data suggest that the results of the LC-MS/MS-OxPCs assay do not replicate the results of two ELISA-based lipid oxidation tests and therefore may produce additional diagnostic information. These findings necessitate development of simplified mass spectrometric procedures for high-throughput and affordable analysis of selected molecular species of OxPCs.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of General Internal Medicine (DAIM) > Geriatric Clinic

UniBE Contributor:

Schoenenberger, Andreas

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0891-5849

Publisher:

Elsevier

Language:

English

Submitter:

Rebecca Rufer

Date Deposited:

30 Jan 2020 15:20

Last Modified:

30 Jan 2020 15:29

Publisher DOI:

10.1016/j.freeradbiomed.2019.05.027

PubMed ID:

31141712

Uncontrolled Keywords:

Biomarkers of hypertension E06 antibody Mass spectrometry of oxidized phospholipids OxLDL

BORIS DOI:

10.7892/boris.138165

URI:

https://boris.unibe.ch/id/eprint/138165

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