A Novel RNA Virus in the Parasitoid Wasp Lysiphlebus fabarum: Genomic Structure, Prevalence, and Transmission

Lüthi, Martina N.; Vorburger, Christoph; Dennis, Alice B. (2020). A Novel RNA Virus in the Parasitoid Wasp Lysiphlebus fabarum: Genomic Structure, Prevalence, and Transmission. Viruses, 12(1) Molecular Diversity Preservation International MDPI 10.3390/v12010059

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We report on a novel RNA virus infecting the wasp Lysiphlebus fabarum, a parasitoid of aphids. This virus, tentatively named “Lysiphlebus fabarum virus” (LysV), was discovered in transcriptome sequences of wasps from an experimental evolution study in which the parasitoids were allowed to adapt to aphid hosts (Aphis fabae) with or without resistance-conferring endosymbionts. Based on phylogenetic analyses of the viral RNA-dependent RNA polymerase (RdRp), LysV belongs to the Iflaviridae family in the order of the Picornavirales, with the closest known relatives all being parasitoid wasp-infecting viruses. We developed an endpoint PCR and a more sensitive qPCR assay to screen for LysV in field samples and laboratory lines. These screens verified the occurrence of LysV in wild parasitoids and identified the likely wild-source population for lab infections in Western Switzerland. Three viral haplotypes could be distinguished in wild populations, of which two were found in the laboratory. Both vertical and horizontal transmission of LysV were demonstrated experimentally, and repeated sampling of laboratory populations suggests that the virus can form persistent infections without obvious symptoms in infected wasps.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Lüthi, Martina Nadine

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

1999-4915

Publisher:

Molecular Diversity Preservation International MDPI

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

29 Jan 2020 15:14

Last Modified:

29 Jan 2020 15:14

Publisher DOI:

10.3390/v12010059

PubMed ID:

31947801

Uncontrolled Keywords:

aphid parasitoid; Lysiphlebus fabarum; RNA virus; viral transmission

BORIS DOI:

10.7892/boris.139338

URI:

https://boris.unibe.ch/id/eprint/139338

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