Detection of Treponema pedis associated with digital dermatitis in Mediterranean buffalo ( Bubalus bubalis )

Guccione, Jacopo; Della Valle, Giovanni; Carcasole, Christian; Kuhnert, Peter; Alsaaod, Maher (2018). Detection of Treponema pedis associated with digital dermatitis in Mediterranean buffalo ( Bubalus bubalis ). Veterinary Record Case Reports, 6(4), e000674. British Veterinary Association 10.1136/vetreccr-2018-000674

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Clinical outcomes compatible with digital dermatitis
(DD) were observed in a second lactation, milking
Mediterranean buffalo (MB). The animal was sharing
the free stall with other 99 milking MBs separated by
a fence from a pen harbouring 40 milking crossbreed
dairy cows. The macroscopic lesion observed on the right
rear limb was categorised as M4.1 stage and associated
with a severe lameness (locomotion score 5). In the
same farm, DD was also observed in 14/40 cows. Nested
PCR analysis of genomic DNA isolated from the centre
lesion’s biopsy showed the presence of Treponema
pedis. Sequence analysis of 494 bp of the PCR fragment
revealed 100 per cent sequence identity to the T pedistype
strain, confirming the PCR result’s specificity and
for the first time the presence of the bacterium in MB. In
order to establish the spread of the DD, a farm inspection
was performed to identify risk factors and preventive
strategies.

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