Cancer Cells Employ Nuclear Caspase-8 to Overcome the p53-Dependent G2/M Checkpoint through Cleavage of USP28.

Müller, Ines; Strozyk, Elwira; Schindler, Sebastian; Beissert, Stefan; Oo, Htoo Zarni; Sauter, Thomas; Lucarelli, Philippe; Raeth, Sebastian; Hausser, Angelika; Al Nakouzi, Nader; Fazli, Ladan; Gleave, Martin E; Liu, He; Simon, Hans-Uwe; Walczak, Henning; Green, Douglas R; Bartek, Jiri; Daugaard, Mads; Kulms, Dagmar (2020). Cancer Cells Employ Nuclear Caspase-8 to Overcome the p53-Dependent G2/M Checkpoint through Cleavage of USP28. Molecular cell, 77(5), 970-984.e7. Cell Press 10.1016/j.molcel.2019.12.023

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Cytosolic caspase-8 is a mediator of death receptor signaling. While caspase-8 expression is lost in some tumors, it is increased in others, indicating a conditional pro-survival function of caspase-8 in cancer. Here, we show that tumor cells employ DNA-damage-induced nuclear caspase-8 to override the p53-dependent G2/M cell-cycle checkpoint. Caspase-8 is upregulated and localized to the nucleus in multiple human cancers, correlating with treatment resistance and poor clinical outcome. Depletion of caspase-8 causes G2/M arrest, stabilization of p53, and induction of p53-dependent intrinsic apoptosis in tumor cells. In the nucleus, caspase-8 cleaves and inactivates the ubiquitin-specific peptidase 28 (USP28), preventing USP28 from de-ubiquitinating and stabilizing wild-type p53. This results in de facto p53 protein loss, switching cell fate from apoptosis toward mitosis. In summary, our work identifies a non-canonical role of caspase-8 exploited by cancer cells to override the p53-dependent G2/M cell-cycle checkpoint.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology

UniBE Contributor:

Liu, He and Simon, Hans-Uwe

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1097-2765

Publisher:

Cell Press

Language:

English

Submitter:

Sabrina Cookman

Date Deposited:

18 Feb 2020 15:01

Last Modified:

08 Mar 2020 01:38

Publisher DOI:

10.1016/j.molcel.2019.12.023

PubMed ID:

31982308

Uncontrolled Keywords:

G2/M checkpoint USP28 apoptosis cancer caspase-8 p53

BORIS DOI:

10.7892/boris.140001

URI:

https://boris.unibe.ch/id/eprint/140001

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