Efficacy of three innovative bacterin vaccines against experimental infection with Mycoplasma hyopneumoniae.

Matthijs, Anneleen Marguerite Filip; Auray, Gaël; Boyen, Filip; Schoos, Alexandra; Michiels, Annelies; García-Nicolás, Obdulio; Barut, Güliz Tuba; Barnier-Quer, Christophe; Jakob, Virginie; Collin, Nicolas; Devriendt, Bert; Summerfield, Artur; Haesebrouck, Freddy; Maes, Dominiek (2019). Efficacy of three innovative bacterin vaccines against experimental infection with Mycoplasma hyopneumoniae. Veterinary research, 50(1), p. 91. BioMed Central 10.1186/s13567-019-0709-0

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New vaccine formulations that include novel strains of Mycoplasma hyopneumoniae and innovative adjuvants designed to induce cellular immunity could improve vaccine efficacy against this pathogen. The aim of this experimental study was to assess the efficacy of three experimental bacterin formulations based on M. hyopneumoniae field strain F7.2C which were able to induce cellular immunity. The formulations included a cationic liposome formulation with the Mincle receptor ligand trehalose 6,6-dibehenate (Lipo_DDA:TDB), a squalene-in-water emulsion with Toll-like receptor (TLR) ligands targeting TLR1/2, TLR7/8 and TLR9 (SWE_TLR), and a poly(lactic-co-glycolic acid) micro-particle formulation with the same TLR ligands (PLGA_TLR). Four groups of 12 M. hyopneumoniae-free piglets were primo- (day (D) 0; 39 days of age) and booster vaccinated (D14) intramuscularly with either one of the three experimental bacterin formulations or PBS. The pigs were endotracheally inoculated with a highly and low virulent M. hyopneumoniae strain on D28 and D29, respectively, and euthanized on D56. The main efficacy parameters were: respiratory disease score (RDS; daily), macroscopic lung lesion score (D56) and log copies M. hyopneumoniae DNA determined with qPCR on bronchoalveolar lavage (BAL) fluid (D42, D56). All formulations were able to reduce clinical symptoms, lung lesions and the M. hyopneumoniae DNA load in the lung, with formulation SWE_TLR being the most effective (RDSD28-D56 -61.90%, macroscopic lung lesions -88.38%, M. hyopneumoniae DNA load in BAL fluid (D42) -67.28%). Further experiments raised under field conditions are needed to confirm these results and to assess the effect of the vaccines on performance parameters.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Auray, Gael; Garcia Nicolas, Obdulio; Barut, Güliz Tuba and Summerfield, Artur

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1297-9716

Publisher:

BioMed Central

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

26 Feb 2020 07:52

Last Modified:

01 Mar 2020 02:50

Publisher DOI:

10.1186/s13567-019-0709-0

PubMed ID:

31703726

BORIS DOI:

10.7892/boris.140303

URI:

https://boris.unibe.ch/id/eprint/140303

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