Coinfection of Swiss cattle with bovine parainfluenza virus 3 and Mycoplasma bovis at acute and chronic stages of bovine respiratory disease complex.

Mehinagic, Kemal; Pilo, Paola; Vidondo, Beatriz; Stokar-Regenscheit, Nadine (2019). Coinfection of Swiss cattle with bovine parainfluenza virus 3 and Mycoplasma bovis at acute and chronic stages of bovine respiratory disease complex. Journal of veterinary diagnostic investigation, 31(5), pp. 674-680. Sage 10.1177/1040638719861686

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Viral agents such as bovine respiratory syncytial virus (BRSV) and bovine parainfluenza virus 3 (BPIV-3) are considered primary infectious agents in bovine respiratory disease complex (BRDC). Information regarding the pathogenesis of BRDC is scarce, especially at an advanced chronicity stage, in addition to ongoing coinfection with other primary agents such as Mycoplasma bovis. Based on a retrospective review of histology slides from 104 autopsy cases, we classified cases according to type of pneumonia and chronicity. We performed immunohistochemistry (IHC) for BRSV, BPIV-3, and M. bovis as well as real-time PCR (rtPCR) for M. bovis on lung tissue of all 104 cases and correlated results with the morphologic type of pneumonia. Histomorphologically, 79 cases were classified as bronchopneumonia, 16 as bronchointerstitial pneumonia, and 9 as interstitial pneumonia. In 89 cases, at least 1 of the investigated agents was detected by IHC; 44 of these cases had a coinfection. BPIV-3 was the predominant agent present, as a single infection in 39 cases, and in coinfection with M. bovis in 39 cases. Comparing the detection methods for M. bovis, rtPCR was more specific and sensitive than IHC. The combination of both methods provided a good visual tool for assessing severity and distribution of M. bovis antigen within the tissue. Unlike BRSV, BPIV-3 and M. bovis persisted in chronic BRDC, suggesting ongoing impairment of defense mechanisms in the lung.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Public Health Institute
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Mehinagic, Kemal; Pilo, Paola; Vidondo, Beatriz and Stokar von Neuforn, Nadine

Subjects:

600 Technology > 630 Agriculture

ISSN:

1943-4936

Publisher:

Sage

Language:

English

Submitter:

Susanne Agnes Lerch

Date Deposited:

26 Feb 2020 08:49

Last Modified:

26 Feb 2020 08:49

Publisher DOI:

10.1177/1040638719861686

PubMed ID:

31246162

Uncontrolled Keywords:

bovine respiratory disease complex bronchopneumonia

BORIS DOI:

10.7892/boris.140591

URI:

https://boris.unibe.ch/id/eprint/140591

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