Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures.

Boonekamp, Kim E; Kretzschmar, Kai; Wiener, Dominique J.; Asra, Priyanca; Derakhshan, Sepideh; Puschhof, Jens; López-Iglesias, Carmen; Peters, Peter J; Basak, Onur; Clevers, Hans (2019). Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures. Proceedings of the National Academy of Sciences of the United States of America - PNAS, 116(29), pp. 14630-14638. National Academy of Sciences NAS 10.1073/pnas.1715272116

[img] Text
b140652.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

Mammalian epidermal stem cells maintain homeostasis of the skin epidermis and contribute to its regeneration throughout adult life. While 2D mouse epidermal stem cell cultures have been established decades ago, a long-term, feeder cell- and serum-free culture system recapitulating murine epidermal architecture has not been available. Here we describe an epidermal organoid culture system that allows long-term, genetically stable expansion of adult epidermal stem cells. Our epidermal expansion media combines atypically high calcium concentrations, activation of cAMP, FGF, and R-spondin signaling with inhibition of bone morphogenetic protein (BMP) signaling. Organoids are established robustly from adult mouse skin and expand over at least 6 mo, while maintaining the basal-apical organization of the mouse interfollicular epidermis. The system represents a powerful tool to study epidermal homeostasis and disease in vitro.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > DermFocus
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology

UniBE Contributor:

Wiener, Dominique Judith

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0027-8424

Publisher:

National Academy of Sciences NAS

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

11 Mar 2020 14:45

Last Modified:

11 Mar 2020 14:45

Publisher DOI:

10.1073/pnas.1715272116

PubMed ID:

31253707

Uncontrolled Keywords:

adult stem cells interfollicular epidermis mammalian epidermis organoids

BORIS DOI:

10.7892/boris.140652

URI:

https://boris.unibe.ch/id/eprint/140652

Actions (login required)

Edit item Edit item
Provide Feedback