Development and validation of an LC-MS/MS method for the bioanalysis of the major metamizole metabolites in human plasma.

Bachmann, Fabio; Blaser, Lea; Haschke, Manuel Martin; Krähenbühl, Stephan; Duthaler, Urs (2020). Development and validation of an LC-MS/MS method for the bioanalysis of the major metamizole metabolites in human plasma. Bioanalysis, 12(3), pp. 175-189. Future Science 10.4155/bio-2019-0251

[img] Text
Bachmann, Bioanalysis 2020.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (853kB) | Request a copy

Aim: Metamizole is a frequently used antipyretic and analgesic prodrug, yet its pharmacokinetics has not been thoroughly studied in infants and with coadministered medications. Thus, an LC-MS/MS method was developed to quantify the four major metamizole metabolites in human plasma. Methodology: Pre- and postcolumn infusion was installed to enable robust analyte retention and electrospray ionization following deproteinization of plasma samples. Results: The method was linear (R > 0.996), accurate (93.1-106.0%) and precise (≤12.7%). Mean recovery was more than 91.8% and ion suppression less than 13.1% for all analytes. Pharmacokinetic profiles were reproducible after 4 years at -80°C except for the formylated metabolite (-22.2%). Conclusion: The method fulfilled pertinent criteria of validation guidelines and required only little sample volume. The method therefore qualifies for metamizole analyses in children.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of General Internal Medicine (DAIM) > Clinic of General Internal Medicine

UniBE Contributor:

Haschke, Manuel Martin

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1757-6180

Publisher:

Future Science

Language:

English

Submitter:

Christine Baumgartner

Date Deposited:

10 Mar 2020 14:33

Last Modified:

10 Mar 2020 14:33

Publisher DOI:

10.4155/bio-2019-0251

PubMed ID:

32052638

Uncontrolled Keywords:

LC–MS/MS bioanalysis dipyrone method validation pediatrics pharmacokinetics

BORIS DOI:

10.7892/boris.140950

URI:

https://boris.unibe.ch/id/eprint/140950

Actions (login required)

Edit item Edit item
Provide Feedback