Baumann, Lisa; Segner, Helmut; Ros, Albert; Knapen, Dries; Vergauwen, Lucia (2019). Thyroid Hormone Disruptors Interfere with Molecular Pathways of Eye Development and Function in Zebrafish. International journal of molecular sciences, 20(7) MDPI 10.3390/ijms20071543
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The effects of thyroid hormone disrupting chemicals (THDCs) on eye development of zebrafish were investigated. We expected THDC exposure to cause transcriptional changes of vision-related genes, which find their phenotypic anchoring in eye malformations and dysfunction, as observed in our previous studies. Zebrafish were exposed from 0 to 5 days post fertilization (dpf) to either propylthiouracil (PTU), a thyroid hormone synthesis inhibitor, or tetrabromobisphenol-A (TBBPA), which interacts with thyroid hormone receptors. Full genome microarray analyses of RNA isolated from eye tissue revealed that the number of affected transcripts was substantially higher in PTU- than in TBBPA-treated larvae. However, multiple components of phototransduction (e.g., phosphodiesterase, opsins) were responsive to both THDC exposures. Yet, the response pattern for the gene ontology (GO)-class "sensory perception" differed between treatments, with over 90% down-regulation in PTU-exposed fish, compared to over 80% up-regulation in TBBPA-exposed fish. Additionally, the reversibility of effects after recovery in clean water for three days was investigated. Transcriptional patterns in the eyes were still altered and partly overlapped between 5 and 8 dpf, showing that no full recovery occurred within the time period investigated. However, pathways involved in repair mechanisms were significantly upregulated, which indicates activation of regeneration processes.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute for Fish and Wildlife Health (FIWI) |
UniBE Contributor: |
Baumann, Lisa Annie, Segner, Helmut |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 630 Agriculture |
ISSN: |
1661-6596 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Pamela Schumacher |
Date Deposited: |
27 Mar 2020 12:10 |
Last Modified: |
07 Aug 2024 15:45 |
Publisher DOI: |
10.3390/ijms20071543 |
PubMed ID: |
30934780 |
Uncontrolled Keywords: |
PTU TBBPA microarray mode of action pathways of toxicity transcriptome analyses |
BORIS DOI: |
10.7892/boris.141722 |
URI: |
https://boris.unibe.ch/id/eprint/141722 |
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