Trace-level determination of two neonicotinoid insecticide residues in honey bee royal jelly using ultra-sound assisted salting-out liquid liquid extraction followed by ultra-high-performance liquid chromatography-tandem mass spectrometry

Giroud, Barbara; Bruckner, Selina; Straub, Lars; Neumann, Peter; Williams, Geoffrey R.; Vulliet, Emmanuelle (2019). Trace-level determination of two neonicotinoid insecticide residues in honey bee royal jelly using ultra-sound assisted salting-out liquid liquid extraction followed by ultra-high-performance liquid chromatography-tandem mass spectrometry. Microchemical journal, 151, p. 104249. Elsevier 10.1016/j.microc.2019.104249

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A few studies have investigated the analysis of neonicotinoid insecticides in honey bee royal jelly. An analytical method based on salting-out liquid-liquid extraction assisted with ultrasound followed by liquid chromatography-tandem mass spectrometry was developed for the determination of thiamethoxam and clothianidin in royal jelly. A cleaning step based on freezing before injection to the UHPLC-MS/MS (MRM mode) allowed maximizing the signal-to-noise ratio. The finalized method was validated in line with European legislation. The limits of quantification (LOQ), reached from a test sample of 100 mg royal jelly only, was 0.25 ng/g for both neonicotinoids. The recoveries were close to 100% with relative standard deviation below 13.7%, measured at three concentration levels. The validated method was finally applied to samples of royal jelly commercially available, and showed the presence of thiamethoxam at levels between the limit of determination (LOD = 0.15 ng/g) and LOQ in a few samples.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Bee Health

UniBE Contributor:

Straub, Lars and Neumann, Peter

Subjects:

500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture

ISSN:

0026-265X

Publisher:

Elsevier

Language:

English

Submitter:

Anna Papach

Date Deposited:

31 Mar 2020 14:46

Last Modified:

05 Apr 2020 02:50

Publisher DOI:

10.1016/j.microc.2019.104249

BORIS DOI:

10.7892/boris.142075

URI:

https://boris.unibe.ch/id/eprint/142075

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