Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models.

Weldearegay, Yenehiwot Berhanu; Müller, Sandy; Hänske, Jana; Schulze, Anja; Kostka, Aline; Rüger, Nancy; Hewicker-Trautwein, Marion; Brehm, Ralph; Valentin-Weigand, Peter; Kammerer, Robert; Jores, Jörg; Meens, Jochen (2019). Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models. Pathogens, 8(2) MDPI 10.3390/pathogens8020082

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Respiratory infections caused by mycoplasma species in ruminants lead to considerable economic losses. Two important ruminant pathogens are Mycoplasma mycoides subsp. Mycoides (Mmm), the aetiological agent of contagious bovine pleuropneumonia and Mycoplasma mycoides subsp. capri (Mmc), which causes pneumonia, mastitis, arthritis, keratitis, and septicemia in goats. We established precision cut lung slices (PCLS) infection model for Mmm and Mmc to study host-pathogen interactions. We monitored infection over time using immunohistological analysis and electron microscopy. Moreover, infection burden was monitored by plating and quantitative real-time PCR. Results were compared with lungs from experimentally infected goats and cattle. Lungs from healthy goats and cattle were also included as controls. PCLS remained viable for up to two weeks. Both subspecies adhered to ciliated cells. However, the titer of Mmm in caprine PCLS decreased over time, indicating species specificity of Mmm. Mmc showed higher tropism to sub-bronchiolar tissue in caprine PCLS, which increased in a time-dependent manner. Moreover, Mmc was abundantly observed on pulmonary endothelial cells, indicating partially, how it causes systemic disease. Tissue destruction upon prolonged infection of slices was comparable to the in vivo samples. Therefore, PCLS represents a novel ex vivo model to study host-pathogen interaction in livestock mycoplasma.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Jores, Jörg

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

2076-0817

Publisher:

MDPI

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

02 Apr 2020 09:35

Last Modified:

02 Apr 2020 09:35

Publisher DOI:

10.3390/pathogens8020082

PubMed ID:

31226867

Uncontrolled Keywords:

3R CBPP MAKePS syndrome Mycoplasma mycoides PCLS immunofluorescence tropism

BORIS DOI:

10.7892/boris.142096

URI:

https://boris.unibe.ch/id/eprint/142096

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