[Whole genome transplantation: bringing natural or synthetic bacterial genomes back to life].

Labroussaa, Fabien; Baby, Vincent; Rodrigue, Sébastien; Lartigue, Carole (2019). [Whole genome transplantation: bringing natural or synthetic bacterial genomes back to life]. médecine/sciences, 35(10), pp. 761-770. EDP Sciences 10.1051/medsci/2019154

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The development of synthetic genomics (SG) allowed the emergence of several groundbreaking techniques including the synthesis, assembly and engineering of whole bacterial genomes. The successful implantation of those methods, which culminated in the creation of JCVI-syn3.0 the first nearly minimal bacterium with a synthetic genome, mainly results from the use of the yeast Saccharomyces cerevisiae as a transient host for bacterial genome replication and modification. Another method played a key role in the resounding success of this project: bacterial genome transplantation (GT). GT consists in the transfer of bacterial genomes cloned in yeast, back into a cellular environment suitable for the expression of their genetic content. While successful using many mycoplasma species, a complete understanding of the factors governing GT will most certainly help unleash the power of the entire SG pipeline to other genetically intractable bacteria.

Item Type:

Journal Article (Review Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Labroussaa, Fabien

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

0767-0974

Publisher:

EDP Sciences

Language:

French

Submitter:

Achim Braun Parham

Date Deposited:

02 Apr 2020 10:00

Last Modified:

02 Apr 2020 10:00

Publisher DOI:

10.1051/medsci/2019154

PubMed ID:

31625898

BORIS DOI:

10.7892/boris.142100

URI:

https://boris.unibe.ch/id/eprint/142100

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