Bovine leptospirosis in abattoirs in Uganda: Molecular detection and risk of exposure among workers.

Alinaitwe, Lordrick; Kankya, Clovice; Allan, Kathryn J; Rodriguez-Campos, Sabrina; Torgerson, Paul; Dreyfus, Anou (2019). Bovine leptospirosis in abattoirs in Uganda: Molecular detection and risk of exposure among workers. Zoonoses and public health, 66(6), pp. 636-646. Wiley 10.1111/zph.12616

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Leptospirosis is a zoonotic bacterial disease reported worldwide. In Uganda, seropositivity has been reported in both humans and domesticated animals, including cattle. However, it remains unknown whether cattle are shedding leptospires and thus acting as potential source for human leptospirosis. We conducted this cross-sectional study in two cattle abattoirs in Kampala, Uganda between June and July 2017. Kidney and urine samples from 500 cattle sourced from across the country were analysed by real-time PCR to establish the prevalence of Leptospira-positive cattle and risk of exposure to abattoir workers. The species of infecting Leptospira was determined by amplification of secY gene and compared to reference sequences published in GenBank. Of 500 cattle tested, 36 (7.2%) had Leptospira DNA in their kidneys (carriers), 29 (5.8%) in their urine (shedders); with an overall prevalence (kidney and/or urine) of 8.8%. Leptospira borgpetersenii was confirmed as the infecting species in three cattle and Leptospira kirschneri in one animal. Male versus female cattle (OR = 3, p-value 0.003), exotic versus local breeds (OR = 21.3, p-value 0.002) or cattle from Western Uganda (OR = 4.4, p-value 0.001) and from regions across the border (OR = 3.3, p-value 0.032) versus from the central region were more likely to be Leptospira-positive. The daily risk of exposure of abattoir workers to ≥1 (kidney and/or urine) positive carcass ranged from 27% (95% credibility interval 18.6-52.3) to 100% (95% CI 91.0-100.0), with halal butchers and pluck inspectors being at highest risk. In conclusion, cattle slaughtered at abattoirs in Uganda carry and shed pathogenic Leptospira species; and this may pose occupation-related risk of exposure among workers in these abattoirs, with workers who handle larger numbers of animals being at higher risk.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Rodriguez, Sabrina

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

1863-2378

Publisher:

Wiley

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

02 Apr 2020 11:12

Last Modified:

02 Apr 2020 11:12

Publisher DOI:

10.1111/zph.12616

PubMed ID:

31250522

Uncontrolled Keywords:

Leptospira abattoir leptospirosis real-time PCR risk of exposure

BORIS DOI:

10.7892/boris.142103

URI:

https://boris.unibe.ch/id/eprint/142103

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