Diabetes affects endothelial cell function and alters fibrin clot formation in a microvascular flow model: A pilot study.

Jenny, Lorenz; Melmer, Andreas; Laimer, Markus; Hardy, Elaissa T; Lam, Wilbur A; Schroeder, Verena (2020). Diabetes affects endothelial cell function and alters fibrin clot formation in a microvascular flow model: A pilot study. Diabetes & vascular disease research, 17(1), p. 1479164120903044. Sage 10.1177/1479164120903044

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Diabetes is a proinflammatory and prothrombotic condition that increases the risk of vascular complications. The aim of this study was to develop a diabetic microvascular flow model that allows to study the complex interactions between endothelial cells, blood cells and plasma proteins and their effects on clot formation. Primary human cardiac microvascular endothelial cells from donors without diabetes or donors with diabetes (type 1 or type 2) were grown in a microfluidic chip, perfused with non-diabetic or diabetic whole blood, and clot formation was assessed by measuring fibrin deposition in real time by confocal microscopy. Clot formation in non-diabetic whole blood was significantly increased in the presence of endothelial cells from donors with type 2 diabetes compared with cells from donors without diabetes. There was no significant difference in clot formation between non-diabetic and diabetic whole blood. We present for the first time a diabetic microvascular flow model as a new tool to study clot formation as a result of the complex interactions between endothelial cells, blood cells and plasma proteins in a diabetes setting. We show that endothelial cells affect clot formation in whole blood, attributing an important role to the endothelium in the development of atherothrombotic complications.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Endocrinology, Diabetology and Clinical Nutrition
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pavillon 52 > Forschungsgruppe Experimentelle Hämostase
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Hämatologie (Erwachsene)

UniBE Contributor:

Jenny, Lorenz; Melmer, Andreas; Laimer, Markus and Schröder, Verena

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1479-1641

Publisher:

Sage

Language:

English

Submitter:

Andrea Stettler

Date Deposited:

27 Mar 2020 14:11

Last Modified:

29 Mar 2020 03:43

Publisher DOI:

10.1177/1479164120903044

PubMed ID:

32037878

Uncontrolled Keywords:

Blood coagulation diabetes mellitus endothelial cells microfluidics

BORIS DOI:

10.7892/boris.142492

URI:

https://boris.unibe.ch/id/eprint/142492

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