Niemann, Moritz; Schneider, André (2020). A Scalable Purification Method for Mitochondria from Trypanosoma brucei. In: Michels, Paul A. M.; Ginger, Michael L.; Zilberstein, Dan (eds.) Trypanosomatids. Methods in Molecular Biology: Vol. 2116 (pp. 611-626). New York, NY: Springer US 10.1007/978-1-0716-0294-2_36
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Due to its unique biology the mitochondrion of Trypanosoma brucei has attracted a lot of interest since many decades, making it arguably the best studied mitochondrion outside yeast and mammals. Here we describe a method allowing purification of mitochondria from procyclic trypanosomes that yields highly enriched and functional organelles. The method is based on isotonic lysis of cells by nitrogen cavitation, DNase I digestion, differential centrifugation and Nycodenz gradient centrifugation. The method is scalable and can be adapted to culture volumes a small as 100 mL or as large as 24 L.
Item Type: |
Book Section (Book Chapter) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Niemann, Moritz, Schneider, André |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry |
ISBN: |
978-1-0716-0293-5 |
Series: |
Methods in Molecular Biology |
Publisher: |
Springer US |
Language: |
English |
Submitter: |
Christina Schüpbach |
Date Deposited: |
07 Apr 2020 08:30 |
Last Modified: |
05 Dec 2022 15:38 |
Publisher DOI: |
10.1007/978-1-0716-0294-2_36 |
BORIS DOI: |
10.7892/boris.142726 |
URI: |
https://boris.unibe.ch/id/eprint/142726 |
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