A Scalable Purification Method for Mitochondria from Trypanosoma brucei

Niemann, Moritz; Schneider, André (2020). A Scalable Purification Method for Mitochondria from Trypanosoma brucei. In: Michels, Paul A. M.; Ginger, Michael L.; Zilberstein, Dan (eds.) Trypanosomatids. Methods in Molecular Biology: Vol. 2116 (pp. 611-626). New York, NY: Springer US 10.1007/978-1-0716-0294-2_36

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Due to its unique biology the mitochondrion of Trypanosoma brucei has attracted a lot of interest since many decades, making it arguably the best studied mitochondrion outside yeast and mammals. Here we describe a method allowing purification of mitochondria from procyclic trypanosomes that yields highly enriched and functional organelles. The method is based on isotonic lysis of cells by nitrogen cavitation, DNase I digestion, differential centrifugation and Nycodenz gradient centrifugation. The method is scalable and can be adapted to culture volumes a small as 100 mL or as large as 24 L.

Item Type:

Book Section (Book Chapter)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Niemann, Moritz and Schneider, André

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISBN:

978-1-0716-0293-5

Series:

Methods in Molecular Biology

Publisher:

Springer US

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

07 Apr 2020 08:30

Last Modified:

28 Jul 2020 20:23

Publisher DOI:

10.1007/978-1-0716-0294-2_36

BORIS DOI:

10.7892/boris.142726

URI:

https://boris.unibe.ch/id/eprint/142726

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