Homologue replacement in the import motor of the mitochondrial inner membrane of trypanosomes

von Känel, Corinne; Muñoz-Gómez, Sergio A; Oeljeklaus, Silke; Wenger, Christoph; Warscheid, Bettina; Wideman, Jeremy G; Harsman, Anke; Schneider, André (2020). Homologue replacement in the import motor of the mitochondrial inner membrane of trypanosomes. eLife, 9 eLife Sciences Publications 10.7554/eLife.52560

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Many mitochondrial proteins contain N-terminal presequences that direct them to the organelle. The main driving force for their translocation across the inner membrane is provided by the presequence translocase-associated motor (PAM) which contains the J-protein Pam18. Here, we show that in the PAM of Trypanosoma brucei the function of Pam18 has been replaced by the non-orthologous euglenozoan-specific J-protein TbPam27. TbPam27 is specifically required for the import of mitochondrial presequence-containing but not for carrier proteins. Similar to yeast Pam18, TbPam27 requires an intact J-domain to function. Surprisingly, T. brucei still contains a bona fide Pam18 orthologue that, while essential for normal growth, is not involved in protein import. Thus, during evolution of kinetoplastids, Pam18 has been replaced by TbPam27. We propose that this replacement is linked to the transition from two ancestral and functionally distinct TIM complexes, found in most eukaryotes, to the single bifunctional TIM complex present in trypanosomes.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

von Känel, Corinne; Wenger, Christoph; Harsman, Anke Judith and Schneider, André

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

2050-084X

Publisher:

eLife Sciences Publications

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

07 Apr 2020 12:23

Last Modified:

08 Apr 2020 01:34

Publisher DOI:

10.7554/eLife.52560

PubMed ID:

32105215

BORIS DOI:

10.7892/boris.142732

URI:

https://boris.unibe.ch/id/eprint/142732

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