Echinococcus multilocularis primary cells: improved isolation, small-scale cultivation and RNA interference

Spiliotis, M.; Mizukami, C.; Oku, Y.; Kiss, F.; Brehm, K.; Gottstein, B. (2010). Echinococcus multilocularis primary cells: improved isolation, small-scale cultivation and RNA interference. Molecular and biochemical parasitology, 174(1), pp. 83-7. Amsterdam: Elsevier 10.1016/j.molbiopara.2010.07.001

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In this study we demonstrate RNA interference mediated knock-down of target gene expression in Echinococcus multilocularis primary cells on both the transcriptional and translational level. In addition, we report on an improved method for generating E. multilocularis primary cell mini-aggregates from in vitro cultivated metacestode vesicles, and on the cultivation of small numbers of small interfering RNA-transfected cells in vitro over an extended period of time. This allows assessments on the effects of RNA interference performed on Echinococcus primary cells with regard to growth, proliferation, differentiation of the parasite and the formation of novel metacestode vesicles in vitro.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology

UniBE Contributor:

Spiliotis, Markus and Gottstein, Bruno

ISSN:

0166-6851

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:36

Last Modified:

23 Jul 2018 12:09

Publisher DOI:

10.1016/j.molbiopara.2010.07.001

PubMed ID:

20637246

Web of Science ID:

000281943300013

BORIS DOI:

10.7892/boris.14302

URI:

https://boris.unibe.ch/id/eprint/14302 (FactScience: 221197)

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