Short- and long-term cultivation of embryonic and neonatal murine keratinocytes

Caldelari, R.; Muller, E.J. (2010). Short- and long-term cultivation of embryonic and neonatal murine keratinocytes. In: Ward, Andrew; Tosh, David (eds.) Mouse Cell Culture. Methods in Molecular Biology: Vol. 633 (pp. 125-138). Heidelberg: Springer Verlag 10.1007/978-1-59745-019-5_10

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Studies using cultured cells allow one to dissect complex cellular mechanisms in greater detail than when studying living organisms alone. However, before cultured cells can deliver meaningful results they must accurately represent the in vivo situation. Over the last three to four decades considerable effort has been devoted to the development of culture media which improve in vitro growth and modeling accuracy. In contrast to earlier large-scale, non-specific screening of factors, in recent years the development of such media has relied increasingly on a deeper understanding of the cell's biology and the selection of growth factors to specifically activate known biological processes. These new media now enable equal or better cell isolation and growth, using significantly simpler and less labor-intensive methodologies. Here we describe a simple method to isolate and cultivate epidermal keratinocytes from embryonic or neonatal skin on uncoated plastic using a medium specifically designed to retain epidermal keratinocyte progenitors in an undifferentiated state for improved isolation and proliferation and an alternative medium to support terminal differentiation.

Item Type:

Book Section (Book Chapter)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology

UniBE Contributor:

Müller, Eliane Jasmine

Series:

Methods in Molecular Biology

Publisher:

Springer Verlag

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:36

Last Modified:

08 Jun 2016 10:31

Publisher DOI:

10.1007/978-1-59745-019-5_10

Web of Science ID:

PREV20100028244

URI:

https://boris.unibe.ch/id/eprint/14306 (FactScience: 221220)

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